rmtОбщая реаниматологияGeneral Reanimatology1813-97792411-7110FSBI "SRIGR" RAMS10.15360/1813-9779-2014-5-27-32rmt-1418Research ArticleТРАВМА. КРОВОПОТЕРЯINJURY. BLOOD LOSSРЕОЛОГИЧЕСКИЕ СВОЙСТВА КРОВИ ПРИ КРОВОПОТЕРЕ (экспериментальное исследование)Blood Rheological Properties in Blood Loss (An experimental study)МанцкаваМ. М.MantskavaM. M.

Грузия, г. Тбилиси, ул. Готуа, 14

14, L. Gotua, Tbilisi, Georgia

mantskavamaka@rambler.ruМомцелидзеН. Г.MomtselidzeN. G.

Грузия, г. Тбилиси, ул. Готуа, 14

14, L. Gotua, Tbilisi, Georgia

ДавлианидзеЛ. Ш.DavlianidzeL. Sh.

Грузия, г. Тбилиси, ул. Готуа, 14

14, L. Gotua, Tbilisi, Georgia

Центр экспериментальной биомедицины им. И. Бериташвили, Тбилиси, ГрузияРоссияI. Beritashvili Center of Experimental Biomedicine, Tbilisi, GeorgiaRussian Federation2014211020141052732Copyright © Манцкава М.М., Момцелидзе Н.Г., Давлианидзе Л.Ш., 20142014Манцкава М.М., Момцелидзе Н.Г., Давлианидзе Л.Ш.Mantskava M.M., Momtselidze N.G., Davlianidze L.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.reanimatology.com/rmt/article/view/1418

Кровопотеря является сложным патологическим процессом с неизученным до конца механизмом. Особое звено, которое задействовано при кровопотерях, это реология крови. Целью нашей работы было комплексное исследование реологических свойств крови при экспериментальной кровопотере различной степени тяжести: агрегируемости и деформируемости эритроцитов, вязкости плазмы, гематокрита.

Материал и методы

Материал и методы. Моделировали кровопотерю разных стадий у наркотизированных животных. Реологические свойства крови изучали с помощью «Georgian technique», метода мембранной фильтрации, «капиллярного метода» и метода стандартного центрифугирования. Анализ данных проводили статистическими программами Оrigin 4.1. (Microcat.Software.Inc) и Microsoft Excel.

Результаты

Результаты. Агрегируемость эритроцитов достоверно увеличивалась по сравнению с контролем на 10, 25, 44%, деформируемость уменьшалась по сравнению с контролем на 12, 13 и 15% при первой, второй, третьей степени кровопотери, соответственно. Дельта вязкости была недостоверной (как в самих подгруппах — при различных степенях кровопотери, так и по сравнению с контролем), гематокрит при кровопотере в сравнении с контролем увеличивался недостоверно.

Заключение

Заключение. Для комплексной оценки степени кровопотери необходима минимальная совокупность гемореологических параметров — показатели агрегируемости и деформируемости эритроцитов, вязкости крови, гематокрита. Основой контроля реологических параметров массивной кровопотери являются особенности агрегации эритроцитов.

Blood loss is a complex pathological process with the mechanism that has not been fully unstudied. Blood rheology is a particular link that is involved in blood losses. Objective: to perform a comprehensive study of blood rheological proper ties, such as erythrocyte aggregability, erythrocyte deformability, plasma viscosity, and packed cell volume, in different degrees of experimental blood loss.

Materials and methods

Materials and methods. Blood loss of different stages was simulated in anesthetized animals. The Georgian technique, membrane filtration, a capillary method, and a standard centrifugation method were used to study blood rheological properties. The data were analyzed applying the statistical programs Origin 4.1 (Microcat.Software.Inc) and Microsoft Excel.

Results

Results. In stages 1, 2, and 3 blood losses, erythrocyte aggregability was significantly increased by 10, 25, and 44% and erythrocyte deformability was decreased by 12, 13, and 15%, respectively, as compared to the controls. The delta viscosity was insignificant (both in the subgroups in different blood loss degreesversus the control group); the packed cell volume in blood loss was increased insignificantly as compared to the control.

Conclusion

Conclusion. Comprehensive assessment of the degree of blood loss requires a minimum set of blood rheological indices, such as erythrocyte aggregability and deformability, blood viscosity, and packed cell volume. The basis for monitoring the rheological indices of massive blood loss is the specific features of erythrocyte aggregation.

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The authors declare that there are no conflicts of interest present.