rmtОбщая реаниматологияGeneral Reanimatology1813-97792411-7110FSBI "SRIGR" RAMS10.15360/1813-9779-2019-6-50-61rmt-1832Research ArticleОБЗОРЫ И КРАТКИЕ СООБЩЕНИЯREVIEWS & SHORT COMMUNICATIONSЛазер-индуцированная флуоресцентная спектроскопия в диагностике тканевой гипоксии (обзор)Laser-Induced Fluorescence Spectroscopy in the Diagnosis of Tissue Hypoxia (Review)БабкинаА. С.BabkinaAnastasiya S.

107031, г. Москва, ул. Петровка, д. 25, стр. 2;117198, г. Москва, ул. Миклухо-Маклая, д. 6

25 Petrovka Str., Build. 2, 107031 Moscow;6 Miklukho-Maсlaya Str., 117198 Moscow

asbabkina@gmail.comНИИ общей реаниматологии им. В. А. Неговского ФНКЦ РР; Российский университет дружбы народовРоссияV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology; Peoples’ Friendship University of RussiaRussian Federation2019241220191565061Copyright © Бабкина А.С., 20192019Бабкина А.С.Babkina A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.reanimatology.com/rmt/article/view/1832

Цель обзора — анализ опыта диагностики тканевой гипоксии методом лазер-индуцированной спектроскопии, а также выявление перспективных направлений и потенциальных возможностей данного метода для дальнейшего его применения в экспериментальной и клинической медицине.

В обзоре представили данные исследований интенсивности флуоресценции эндогенных молекул-флуорофоров (никотинадениндинуклеотида восстановленного, флавинадениндинуклеотида окисленного) как маркеров ишемического повреждения внутренних органов (мозга, сердца, печени, почек и др.). Рассмотрели принципы метода флуоресцентной лазер-индуцированной спектроскопии in vivo. Уделили внимание историческим аспектам темы. Показали результаты применения метода в экспериментальных и клинических исследованиях тканевой гипоксии и ишемии. Выявили трудности в интерпретации значений интенсивности аутофлуоресцентного сигнала исследуемых молекул. Отметили, что неизвестным остается период сохранения способности ткани к аутофлуоресценции в условиях длительной аноксии, отсутствуют систематизированные представления о влиянии экзогенных и эндогенных факторов на интенсивность аутофлуоресценции.

Сделали вывод, что применение метода лазер-индуцированной флуоресцентной спектроскопии с целью диагностики тканевой ишемии является перспективным направлением в экспериментальной и клинической медицине, не исчерпавшим себя, и оставляющим ряд нерешенных вопросов, несмотря на большое количество исследований в данной области.

The aim of review is to discuss the results of studies on diagnosis of tissue hypoxia by laser-induced spectroscopy, as well as to identify promising trends and prospects of this technique for its further application in experimental and clinical medicine.

The review presents the findings of studies of the fluorescence intensity of endogenous fluorophore molecules (reduced nicotinamide adenine dinucleotide, oxidized flavin adenine dinucleotide) as markers of ischemic injury of internal organs (brain, heart, liver, kidneys, etc.). The principles of fluorescence laser-induced spectroscopy in vivo are discussed. The historical aspects of the subject are also covered. The results of the use of the technique in experimental and clinical studies of tissue hypoxia and ischemia are shown. Difficulties in interpreting the intensity values of autofluorescent signal of the studied molecules are revealed. It was noted that the tissue autofluorescence in a long-term anoxia remains unknown, and there are no structured ideas about the impact of exogenous and endogenous factors on autofluorescence intensity.

In conclusion, the use of laser-induced fluorescence spectroscopy to diagnose tissue ischemia is a promising area of experimental and clinical medicine, which still has various unresolved issues, despite a large number of studies in this domain.

лазер-индуцированная флуоресценцияНАДНФАДгипоксияэндогенные флуорофорытканевая гипоксияишемия головного мозгаlaser-induced fluorescenceNADHFADhypoxiaendogenous fluorophorestissue hypoxiabrain ischemiaАвтор искренне признателен д. м. н., профессору А. М. Голубеву (НИИ общей реаниматологии им. В. А. Неговского ФНКЦ РР) за помощь в написании обзора и сделанные ценные замечания.The author would like to express her sincere gratitude to professor A. M. Golubev, MD, PhD, DSci (V. A. Negovsky Scientific Research Institute of General Reanimatology) for his help in writing this review and for his valuable comments.ReferencesАбрамова Е.А., Военнов О.В., Бояринов Г.А., Трофимов А.О. Церебральная циркуляция и метаболизм у пострадавших с черепномозговой травмой. Общая реаниматология. 2018; 14 (1): 4–11. DOI: 10.15360/1813-9779-2018-1-4-11Abramova E.A., Voennov O.V., Boyarinov G.A., Trofimov А.O. Cerebral Circulation and Metabolism of Patients with Cerebral Injury. 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The authors declare that there are no conflicts of interest present.