rmtОбщая реаниматологияGeneral Reanimatology1813-97792411-7110FSBI "SRIGR" RAMS10.15360/1813-9779-2020-4-4-13rmt-1938Research ArticleКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ И ПРАКТИКАCLINICAL STUDIES AND PRACTICEВлияние переливания свежезамороженной плазмы на электрохимические параметры плазмы крови пациентов с тяжелой сочетанной травмойEffect of Fresh Frozen Plasma Transfusion on Electrochemical Parameters of Blood Plasma in Patients with Severe Combined TraumaГорончаровскаяИ. В.GoroncharovskayaI. V.

Ирина Викторовна Горончаровская  

129090, г. Москва, Большая Сухаревская пл., д. 3, стр. 1

 

Irina V. Goroncharovskaya

goririna22@gmail.comЕвсеевА. К.EvseevA. K.

3 Bolshaya Sukharevskaya Square, 129090 Moscow

ШабановА. К.ShabanovA. K.

129090, г. Москва, Большая Сухаревская пл., д. 3, стр. 1; 

107031, г. Москва, ул. Петровка, дом 25, стр. 2

3 Bolshaya Sukharevskaya Square, 129090 Moscow; 

25 Petrovka Str., Bldg. 2, 107031 Moscow

 

КулабуховВ. В.KulabukhovV. V.

3 Bolshaya Sukharevskaya Square, 129090 Moscow

КузовлевА. Н.KuzovlevA. N.ПопугаевК. А.PopugaevK. A.

3 Bolshaya Sukharevskaya Square, 129090 Moscow

ПетриковС. С.PetrikovS. S.

3 Bolshaya Sukharevskaya Square, 129090 Moscow

НИИ скорой помощи им. Н. В. Склифосовского ДЗМРоссияN. V. Sklifosovsky Research Institute for Emergency Medicine, Moscow Healthcare DepartmentRussian FederationНИИ скорой помощи им. Н. В. Склифосовского ДЗМ; НИИ общей реаниматологии им. В. А. Неговского ФНКЦ РРРоссияN. V. Sklifosovsky Research Institute for Emergency Medicine, Moscow Healthcare Department; V. A. Negovsky Research Institute of General Reanimatology,Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyRussian FederationНИИ общей реаниматологии им. В. А. Неговского ФНКЦ РРРоссияV. A. Negovsky Research Institute of General Reanimatology,Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyRussian Federation202028082020164413Copyright © Горончаровская И.В., Евсеев А.К., Шабанов А.К., Кулабухов В.В., Кузовлев А.Н., Попугаев К.А., Петриков С.С., 20202020Горончаровская И.В., Евсеев А.К., Шабанов А.К., Кулабухов В.В., Кузовлев А.Н., Попугаев К.А., Петриков С.С.Goroncharovskaya I.V., Evseev A.K., Shabanov A.K., Kulabukhov V.V., Kuzovlev A.N., Popugaev K.A., Petrikov S.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.reanimatology.com/rmt/article/view/1938Цель исследования

Цель исследования: изучить изменения электрохимических параметров плазмы крови пациентов с тяжелой сочетанной травмой до и после трансфузий свежезамороженной плазмы (СЗП).

Материалы и методы

Материалы и методы. Исследовали потенциал при разомкнутой цепи (ПРЦ) платинового электрода и антиоксидантную активность плазмы крови 35-и пациентов с тяжелой сочетанной травмой и 35-и образцов СЗП сроком хранения не менее 6 месяцев. Исследование электрохими- ческих параметров плазмы крови пациентов проводили до трансфузии, через 1 час и 24 часа после трансфузии.

Результаты

Результаты. Обнаружили, что величины ПРЦ в СЗП были положительнее по сравнению величи- нами ПРЦ, измеренными в плазме крови реципиентов в 34 из 35 случаев (97%). Показали, что у паци- ентов с тяжелой сочетанной травмой происходит повышение ПРЦ с 5,047 [-7,553; 12,976] мВ до 12,827 [-1,372; 24,764] мВ, а также снижение антиоксидантной активности через 24 часа после транс- фузии СЗП с 16,979 [11,302; 20,946] мкКл до 13,551 [9,288; 18,405] мкКл. Выявили отсутствие значимых изменений клинических показателей крови после трансфузии СЗП.

Заключение

Заключение. С помощью измерений электрохимических параметров плазмы крови пациентов с тяжелой сочетанной травмой до и после трансфузии СЗП, выявили, что, несмотря на отсутствие из- менений в параметрах крови, определяемых рутинными методами, происходят изменения в состоя- нии антиоксидантной системы организма, выражающиеся в смещении величины ПРЦ в плазме крови пациентов в область более положительных значений и снижении антиоксидантной активно- сти. Нарушение окислительно-восстановительного баланса организма может быть причиной разви- тия окислительного стресса.

Purpose

Purpose: to study the dynamics of blood plasma electrochemical parameters in patients with severe combined trauma before and after fresh frozen plasma (FFP) transfusions.

Materials and methods

Materials and methods. The open circuit potential (OCP) of platinum electrode and antioxidant activity of blood plasma were studied in 35 patients with severe combined trauma and 35 post-FFP samples with at least 6-month shelf life. The electrochemical parameters of patients’ blood plasma were analyzed before transfusion, and 1 hr. and 24 hrs. after transfusion.

Results

Results. OCP measured in FFP was found to be more positive vs. OCP measured in recipients' blood plasma in 34 out of 35 cases (97%). It has been shown that in patients with severe combined trauma, OCP increased from 5.047 [-7.553; 12.976] mV to 12.827 [-1.372; 24.764] mV and antioxidant activity decreased 24 hours after FFP transfusion from 16.979 [11.302; 20.946] µC to 13.551 [9.288; 18.405] µC. After FFP transfusion, there were no significant changes in clinical blood parameters.

Conclusion

Conclusion. By measuring electrochemical parameters of blood plasma in patients with severe combined trauma before and after FFP transfusions, it was discovered that in spite of absence of changes in blood parameters by routine methods, there are changes in the condition of the antioxidant system of the body, which manifest in the bias of patients’ blood plasma OCP towards higher positive values and decreased antioxidant activity. Redox imbalance in the body might cause the oxidative stress development.

потенциал при разомкнутой цепиплатиновый электродтрансфузияанти- оксидантная активностьтяжелая сочетанная травмаплазма кровиopen circuit potentialplatinum electrodetransfusionantioxidant activitysevere combined traumablood plasmaReferencesStanworth S.J.,Walsh T.S., Prescott R.J., Lee R.J.,Watson D.M.,Wyncoll, D., Intensive Care Study of Coagulopathy (ISOC) investigators. A national study of plasma use in critical care: clinical indications, dose and effect on prothrombin time. Crit. Care. 2011; 15 (2): R108. DOI: 10.1186/cc10129. PMID: 21466676Stanworth S.J.,Walsh T.S., Prescott R.J., Lee R.J.,Watson D.M.,Wyncoll, D., Intensive Care Study of Coagulopathy (ISOC) investigators. A national study of plasma use in critical care: clinical indications, dose and effect on prothrombin time. Crit. Care. 2011; 15 (2): R108. DOI: 10.1186/cc10129. PMID: 21466676Norfolk D. (eds.). Handbook of Tranfusion Medicine. United Kingdom Blood Services. Norwich: TSO information & publishing solutions; 2013: 184. ISBN 978-0117068469.Norfolk D. (eds.). Handbook of Tranfusion Medicine. United Kingdom Blood Services. Norwich: TSO information & publishing solutions; 2013: 184. ISBN 978-0117068469.Vincent J.L., Piagnerelli M. Transfusion in the intensive care unit. Crit. Care Med. 2006; 34 (5 Suppl): S96–S101. DOI: 10.1097/01.CCM.0000214314.57109.CD. PMID: 16617264.Vincent J.L., Piagnerelli M. Transfusion in the intensive care unit. Crit. Care Med. 2006; 34 (5 Suppl): S96–S101. DOI: 10.1097/01.CCM. 0000214314.57109.CD. PMID: 16617264.Хубутия М.Ш., Шабанов А.К. Основные причины летальности у пострадавших с тяжелой сочетанной травмой в отделении реанимации. Скорая медицинская помощь. 2010; 11 (3): 64–69.Khubutiya M.Sh., Shabanov A.K. The main causes of mortality in patients with severe concomitant trauma in the intensive care unit. Skoraya meditsinskaya pomoshch. 2010; 11 (3): 64–69 [In Russ.].Bhananker S.M., Ramaiah R. Trends in trauma transfusion. Int. J. Crit. Illn. Inj. Sci. 2011; 1 (1): 51–56. DOI: 10.4103/2229-5151.79282. PMID: 22096774.Bhananker S.M., Ramaiah R. Trends in trauma transfusion. Int. J. Crit. Illn. Inj. Sci. 2011; 1 (1): 51–56. DOI: 10.4103/2229-5151.79282. PMID: 22096774.Prakash D. Anemia in the ICU: anemia of chronic disease versus anemia of acute illness. Crit. Care Clin. 2012; 28 (3): 333–343. DOI: 10.1016/j.ccc.2012.04.012. PMID: 22713609.Prakash D. Anemia in the ICU: anemia of chronic disease versus anemia of acute illness. Crit. Care Clin. 2012; 28 (3): 333–343. DOI: 10.1016/j.ccc.2012.04.012. PMID: 22713609.Thomas J., Jensen L., Nahirniak S., Gibney R.T.N. Anemia and blood transfusion practices in the critically ill: A prospective cohort review. Heart Lung. 2010; 39 (3): 217–225. DOI: 10.1016/j.hrtlng.2009.07.002. PMID: 20457342.Thomas J., Jensen L., Nahirniak S., Gibney R.T.N. Anemia and blood transfusion practices in the critically ill: A prospective cohort review. Heart Lung. 2010; 39 (3): 217–225. DOI: 10.1016/j.hrtlng.2009.07.002. PMID: 20457342.Davenport R.A., Brohi K. Cause of trauma-induced coagulopathy. Curr. Opin. Anaesthesiol. 2016; 29 (2): 212–219. DOI: 10.1097/ACO.0000000000000295. PMID: 26705132.Davenport R.A., Brohi K. Cause of trauma-induced coagulopathy. Curr. Opin. Anaesthesiol. 2016; 29 (2): 212–219. DOI: 10.1097/ACO. 0000000000000295. PMID: 26705132.Йовенко И.А., Кобеляцкий Ю.Ю., Царев А.В., Кузьмова Е.А., Клименко К.А., Дубовская Л.Л., Селезнева У.В. Интенсивная терапия кровопотери, коагулопатии и гиповолемического шока при политравме. Медицина неотложных состояний. 2016; 4 (75): 64–71. DOI: 10.22141/2224-0586.4.75.2016.75819.Jovenko I.A., Kobelyackij Yu.Yu., Tsarev A.V., Kuzmova E.A., Klimenko K.A., Dubovskaya L.L., Selezneva U.V. Intensive therapy of blood loss, coagulopathy and hypovolemic shock in polytrauma. Meditsina neotlozhnyh sostoyanij. 2016; 4 (75): 64–71 [In Russ.]. DOI: 10.22141/2224-0586.4.75.2016.75819.Протопопова Е.Б., Мадзаев С.Р., Султанбаев У.С., Зарубин М.В., Файбушевич А.Г., Жибурт Е.Б. Новое в доказательном переливании эритроцитов. Вестник Национального медико-хирургического центра им. Н.И. Пирогова. 2015; 10 (1): 56–58.Protopopova E.B., Madzaev S.R., Sultanbaev U.S., Zarubin M.V., Fajbushevich A.G., Zhiburt E.B. New in evidence-based erythrocyte transfusion. Vestnik Natsionalnogo mediko-khirurgicheskogo centra im. N.I. Pirogova. 2015; 10 (1): 56–58 [In Russ.].Чикаев В.Ф., Вдовин В.А., Галяутдинов Ф.Ш., Ибрагимов Р.А. Особенности инфузионно-трансфузионной терапии в комплексном лечении пострадавших с сочетанной травмой. Казанский медицинский журнал. 2015; 96 (3): 448–451. DOI: 1017750/KMJ2015-448.Chikaev V.F., Vdovin V.A., Galyautdinov F.SH., Ibragimov R.A. The features of infusion-transfusion therapy in the complex treatment of patients with combined trauma. Kazanskij meditsinskij zhurnal. 2015; 96 (3): 448–451 [In Russ.]. DOI: 1017750/KMJ2015-448.Приказ Министерства здравоохранения Российской Федерации (Минздрав России) от 2 апреля 2013 г. N 183н г. Москва «Об утверждении правил клинического использования донорской крови и (или) ее компонентов». Российская газета — Федеральный выпуск. 2013; 190 (6166).Order of the Ministry of Health of the Russian Federation (Ministry of Health of Russia) of April 2, 2013 N 183n, Moscow «On the approval of the rules for the clinical use of donor blood and (or) its components.» Rossijskaya gazeta — Federalnyj vypusk. 2013; 190 (6166) [In Russ.]Кровь донорская и ее компоненты. Руководство по применению компонентов донорской крови. ГОСТ Р 53470-2009. М.: Стандартинформ; 2010: 71.Donor blood and its components. Guidelines for the use of donor blood components. GOST R 53470-2009. M.: Standartinform. 2010: 71. [In Russ.]Spahn D.R., Bouillon B., Cerny V., Duranteau J., Filipescu D., Hunt B.J., Komadina R., Maegele M., Nardi G., Riddez L., Samama C-M.,Vincent J-L., Rossaint R. The European guideline on management of major bleeding and coagulopathy following trauma: fifth edition. Crit. Care. 2019; 23 (1): 98. DOI: 10.1186/s13054-019-2347-3. PMID: 30917843.Spahn D.R., Bouillon B., Cerny V., Duranteau J., Filipescu D., Hunt B.J., Komadina R., Maegele M., Nardi G., Riddez L., Samama C-M.,Vincent J-L., Rossaint R. The European guideline on management of major bleeding and coagulopathy following trauma: fifth edition. Crit. Care. 2019; 23 (1): 98. DOI: 10.1186/s13054-019-2347-3. PMID: 30917843.Постановление Правительства РФ от 26.01.2010 N 29 «Об утверждении технического регламента о требованиях безопасности крови, ее продуктов, кровезамещающих растворов и технических средств, используемых в трансфузионно-инфузионной терапии» URL: https://base.garant.ru/ [Дата обращения 16 апреля 2020 г.]Decree of the Government of the Russian Federation of January 26, 2010 N 29 «On the approval of technical regulations on the safety requirements of blood, its products, blood substitute solutions and technical means used in transfusion-infusion therapy» URL: https://base.garant.ru/ [ Date of treatment April 16, 2020] [In Russ.]Постановление Правительства РФ от 22.06.2019 N 797 «Об утверждении Правил заготовки, хранения, транспортировки и клинического использования донорской крови и ее компонентов и о признании утратившими силу некоторых актов Правительства Российской Федерации». URL: https://base.garant.ru/72284110/ [Дата обращения 16 апреля 2020 г.]Decree of the Government of the Russian Federation of June 22, 2019 N 797 «On approval of the Rules for the procurement, storage, transportation and clinical use of donor blood and its components and on the recognition as invalid of some acts of the Government of the Russian Federation.» URL: https: //base.garant.ru/72284110/ [Retrieved April 16, 2020] [In Russ.]Guide to the preparation, use and quality assurance of blood components. European Directorate for the Quality of Medicines & HealthCare of the Council of Europe (EDQM). Strasbourg: EDQM Publishers; 2017: 540. ISBN 978-92-871-8415-3.Guide to the preparation, use and quality assurance of blood components. European Directorate for the Quality of Medicines & HealthCare of the Council of Europe (EDQM). Strasbourg: EDQM Publishers; 2017: 540. ISBN 978-92-871-8415-3.Green L., Bolton-Maggs P., Beattie C., Cardigan R., Kallis Y., Stanworth S.J., Thachil J., Zahra S. British Society of Haematology Guidelines on the spectrum of fresh frozen plasma and cryoprecipitate products: their handling and use in various patient groups in the absence of major bleeding. Br. J. Haematol. 2018; 181 (1): 54–67. DOI: 10.1111/bjh.15167. PMID: 29527654.Green L., Bolton-Maggs P., Beattie C., Cardigan R., Kallis Y., Stanworth S.J., Thachil J., Zahra S. British Society of Haematology Guidelines on the spectrum of fresh frozen plasma and cryoprecipitate products: their handling and use in various patient groups in the absence of major bleeding. Br. J. Haematol. 2018; 181 (1): 54–67. DOI: 10.1111/bjh.15167. PMID: 29527654.Аксельрод Б.А., Балашова Е.Н., Баутин А.Е., Баховадинов Б.Б., Бирюкова Л.С., Буланов А.Б., Быстрых О.А., Виноградова М.А., Галстян Г.М., Гапонова Т.В., Головкина Л.Л., Гороховский В.С., Еременко А.А., Жибурт Е.Б., Журавель С.В., Кохно А.В., Кузьмина Л.А., Кулабухов В.В., Купряшов А.А., Лубнин А.Ю., Мазурок В.А., Меньшугин И.Н., Минеева Н.В., Михайлова Е.А., Никитин Е.А., Оловникова Н.И., Ошоров А.В., Певцов Д.Э., Попцов В.Н., Рогачевский О.В., Салимов Э.Л., Титков К.В., Трахтман П.Е., Троицкая В.В., Федорова Т.А., Фидарова З.Т., Цветаева Н.В., Чжао А.В., Шестаков Е.Ф. Клиническое использование эритроцитсодержащих компонентов донорской крови. Гематология и трансфузиология. 2018; 63 (4): 372–435. DOI: 10.25837/HAT.2019.62.39.006.Akselrod B. A., Balashova E. N., Bautin A. E., Bahovadinov B. B., Biryukova L. S., Bulanov A. B., Fast O. A., Vinogradova M. A., Galstyan G. M., Gaponova T. V., Golovkina L. L., Gorokhovsky V. S., Eremenko A. A., Zhiburt E. B., Zhuravel S.V., Kohno A.V., Kuzmina L. A., Kulabukhov V.V., Kupriashov A. A., Lubnin A.Yu., Mazurok V. A., Menshugin I. N., Mineeva N. V., Mikhailova E. A., Nikitin E. A., Olovnikova N. I., Oshorov A.V., Pevtsov D. E., Poptsov V. N., Rogachevsky O.V., Salimov E. L., Titkov K.V., Trakhtman P. E., Troitskaya V.V., Fedorova T. A., Fidarova Z. T., Tsvetaeva N. V., Zhao A.V., Shestakov E. F. Clinical use of erythrocyte-containing components of donor blood. Gematologiya i transfuziologiya. 2018; 63 (4): 372–435 [In Russ.]. DOI: 10.25837/HAT.2019.62.39.006.Manual on the management, maintenance and use of blood cold chain equipment. Geneva: World Health Organization; 2005: 92. ISBN 9241546735.Manual on the management, maintenance and use of blood cold chain equipment. Geneva: World Health Organization; 2005: 92. ISBN 9241546735.Wang D., Sun J., Solomon S.B., Klein H.G., Natanson C. Transfusion of older stored blood and risk of death: a meta-analysis. Transfusion. 2012; 52 (6): 1184–1195. DOI: 10.1111/j.1537-2995.2011.03466.x. PMID: 22188419.Wang D., Sun J., Solomon S.B., Klein H.G., Natanson C. Transfusion of older stored blood and risk of death: a meta-analysis. Transfusion. 2012; 52 (6): 1184–1195. DOI: 10.1111/j.1537-2995.2011.03466.x. PMID: 22188419.Bhaduri B., Kandel M., Brugnara C., Tangella K., Popescu G. Optical assay of erythrocyte function in banked blood. Sci. Rep. 2014; 4: 6211. DOI: 10.1038/srep06211. PMID: 25189281.Bhaduri B., Kandel M., Brugnara C., Tangella K., Popescu G. Optical assay of erythrocyte function in banked blood. Sci. Rep. 2014; 4: 6211. DOI: 10.1038/srep06211. PMID: 25189281.Koch C.G., Figueroa P.I., Li L., Sabik J.F. III, Mihaljevic T., Blackstone E.H. Red blood cell storage: how long is too long? Ann. Thorac. Surg. 2013; 96 (5): 1894–1899. DOI: 10.1016/j.athoracsur.2013.05.116. PMID: 24090578.Koch C.G., Figueroa P.I., Li L., Sabik J.F. III, Mihaljevic T., Blackstone E.H. Red blood cell storage: how long is too long? Ann. Thorac. Surg. 2013; 96 (5): 1894–1899. DOI: 10.1016/j.athoracsur.2013.05.116. PMID: 24090578.Henkelman S., Noorman F., Badloe J.F., Lagerberg, J.W. Utilization and quality of cryopreserved red blood cells in transfusion medicine. Vox Sang. 2015; 108 (2): 103–112. DOI: 10.1111/vox.12218. PMID: 25471135.Henkelman S., Noorman F., Badloe J.F., Lagerberg, J.W. Utilization and quality of cryopreserved red blood cells in transfusion medicine. Vox Sang. 2015; 108 (2): 103–112. DOI: 10.1111/vox.12218. PMID: 25471135.Chang A.L., Hoehn R.S., Jernigan P., Cox D., Schreiber M., Pritts T.A. Previous cryopreservation alters the natural history of the red blood cell storage lesion. Shock. 2016; 46 (3 Suppl 1): 89–95. DOI: 10.1097/SHK.0000000000000668. PMID: 27380532.Chang A.L., Hoehn R.S., Jernigan P., Cox D., Schreiber M., Pritts T.A. Previous cryopreservation alters the natural history of the red blood cell storage lesion. Shock. 2016; 46 (3 Suppl 1): 89–95. DOI: 10.1097/SHK.0000000000000668. PMID: 27380532.Берковский А.Л., Ватагина Е.А., Сергеева Е.В., Суворов А.В., Алексанян М.Ж. К вопросу о качестве свежезамороженной плазмы. Гематология и трансфузиология. 2012; 57 (4): 32–34Berkovskij A.L.,Vatagina E.A., Sergeeva E.V., Suvorov A.V., Aleksanyan M.Zh. On the issue of the quality of fresh frozen plasma. Gematologiya i transfuziologiya. 2012; 57 (4): 32–34. [In Russ.]Рагимов А.А., Щербакова Г.Н. Инфузионно-трансфузионная терапия. М: ГЭОТАР-Медиа; 2017: 256. ISBN 978-5-9704-4020-9.Ragimov A.A., Shcherbakova G.N. Infusion-transfusion therapy. M: GEOTAR-Media; 2017: 256. ISBN 978-5-9704-4020-9.Acker J.P., Marks D.C., Sheffield W.P. Quality Assessment of Established and Emerging Blood Components for Transfusion. J. Blood Transfus. 2016; 2016: 4860284. DOI: 10.1155/2016/4860284. PMID: 28070448.Acker J.P., Marks D.C., Sheffield W.P. Quality Assessment of Established and Emerging Blood Components for Transfusion. J. Blood Transfus. 2016; 2016: 4860284. DOI: 10.1155/2016/4860284. PMID: 28070448.Hess J.R. Red cell changes during storage. Transfus. Apher. Sci. 2010; 43 (1): 51–59. DOI: 10.1016/j.transci.2010.05.009. PMID: 20558107.Hess J.R. Red cell changes during storage. Transfus. Apher. Sci. 2010; 43 (1): 51–59. DOI: 10.1016/j.transci.2010.05.009. PMID: 20558107.Kirkpatrick U.J., Adams R.A., Lardi A., McCollum C.N. Rheological properties and function of blood cells in stored bank blood and salvaged blood. Br. J. Haematol. 1998; 101 (2): 364–368. DOI: 10.1046/j.1365-2141.1998.00689.x. PMID: 9609536.Kirkpatrick U.J., Adams R.A., Lardi A., McCollum C.N. Rheological properties and function of blood cells in stored bank blood and salvaged blood. Br. J. Haematol. 1998; 101 (2): 364–368. DOI: 10.1046/j.1365-2141.1998.00689.x. PMID: 9609536.Luten M., Roerdinkholder-Stoelwinder B., Schaap N.P., De Grip W.J., Bos H.J., Bosman G.J. Survival of red blood cells after transfusion: a comparison between red cells concentrates of different storage periods. Transfusion. 2008; 48 (7): 1478–1485. DOI: 10.1111/j.15372995.2008.01734.x. PMID: 18482180.Luten M., Roerdinkholder-Stoelwinder B., Schaap N.P., De Grip W.J., Bos H.J., Bosman G.J. Survival of red blood cells after transfusion: a comparison between red cells concentrates of different storage periods. Transfusion. 2008; 48 (7): 1478–1485. DOI: 10.1111/j.15372995.2008.01734.x. PMID: 18482180.Manchenko E.A., Kozlova E.K., Sergunova V.A., Chernysh A.M. Homogeneous Deformation of Native Erythrocytes During Long-Term Storage. General Reanimatology. 2019; 15 (5): 4–10. DOI: 10.15360/1813-9779-2019-5-4-10.Manchenko E.A., Kozlova E.K., Sergunova V.A., Chernysh A.M. Homogeneous Deformation of Native Erythrocytes During Long-Term Storage. General Reanimatology. 2019; 15 (5): 4–10. DOI: 10.15360/1813-9779-2019-5-4-10.Illert W.E., Butsch H., Nuber D., Howe J., Sänger W.,Weidinger S. LongTerm Storage of Fresh Frozen Plasma at –40° C. A Multicenter Study on the Stability of Labile Coagulation Factors over a Period of 3 Years. Transf. Med. Hemoth. 2001; 28 (4): 189–194. DOI: 10.1159/000050236.Illert W.E., Butsch H., Nuber D., Howe J., Sänger W.,Weidinger S. LongTerm Storage of Fresh Frozen Plasma at –40° C. A Multicenter Study on the Stability of Labile Coagulation Factors over a Period of 3 Years. Transf. Med. Hemoth. 2001; 28 (4): 189–194. DOI: 10.1159/000050236.Goroncharovskaya I.V., Khvatov V.B., Evseev A.K., Shabanov A.K., Goldin M.M., Petrikov S.S. Monitoring of the Blood Plasma Redox Potential During Plasma Quarantining (Preliminary Report). General Reanimatology. 2019; 15 (1): 47–53. DOI: 10.15360/1813-9779-2019-1-47-53.Goroncharovskaya I.V., Khvatov V.B., Evseev A.K., Shabanov A.K., Goldin M.M., Petrikov S.S. Monitoring of the Blood Plasma Redox Potential During Plasma Quarantining (Preliminary Report). General Reanimatology. 2019; 15 (1): 47–53. DOI: 10.15360/1813-9779-2019-1-47-53.Kriebardis A.G., Antonelou M.H., Georgatzakou H.T., Tzounakas V.L., Stamoulis K.E., Papassideri I.S. Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects. Blood Transfus. 2016; 14 (2): 228–237. DOI: 10.2450/2016.0179-15. PMID: 27136430.Kriebardis A.G., Antonelou M.H., Georgatzakou H.T., Tzounakas V.L., Stamoulis K.E., Papassideri I.S. Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects. Blood Transfus. 2016; 14 (2): 228–237. DOI: 10.2450/2016.0179-15. PMID: 27136430.Stratford N. Antioxidant potential of iv fluids. Br. J. Anaesth. 1997; 78 (6): 757–759. DOI: 10.1093/bja/78.6.757. PMID: 9215032.Stratford N. Antioxidant potential of iv fluids. Br. J. Anaesth. 1997; 78 (6): 757–759. DOI: 10.1093/bja/78.6.757. PMID: 9215032.Chevion S., Roberts M.A., Chevion M. The use of cyclic voltammetry for the evaluation of antioxidant capacity. Free Radic. Biol. Med. 2000; 28 (6): 860–870. DOI: 10.1016/s0891-5849 (00)00178-7. PMID: 10802216.Chevion S., Roberts M.A., Chevion M. The use of cyclic voltammetry for the evaluation of antioxidant capacity. Free Radic. Biol. Med. 2000; 28 (6): 860–870. DOI: 10.1016/s0891-5849(00)00178-7. PMID: 10802216.Psotova J.,Zahalkova J., Hrbac J., Simanek V., Bartek J. Determination of total antioxidant capacity in plasma by cyclic voltammetry. Two case reports. Biomed. Pap. Med. Fac. Univ. Palacky. Olomouc. Czech. Repub. 2001; 145 (2): 81–83. PMID: 12426779Psotova J.,Zahalkova J., Hrbac J., Simanek V., Bartek J. Determination of total antioxidant capacity in plasma by cyclic voltammetry. Two case reports. Biomed. Pap. Med. Fac. Univ. Palacky. Olomouc. Czech. Repub. 2001; 145 (2): 81–83. PMID: 12426779Tsivadze A.Yu., Petrikov S.S., Goroncharovskaya I.V., Evseev A.K., Shabanov A.K., Batishchev O.V., Andreev V.N., Goldin M.M. Voltammetric Analysis in Blood Serum in Patients with Severe Combined Trauma. Dokl. Phys. Chem. 2019; 486 (1): 67–69. DOI: 10.1134/S0012501619050014.Tsivadze A.Yu., Petrikov S.S., Goroncharovskaya I.V., Evseev A.K., Shabanov A.K., Batishchev O.V., Andreev V.N., Goldin M.M. Voltammetric Analysis in Blood Serum in Patients with Severe Combined Trauma. Dokl. Phys. Chem. 2019; 486 (1): 67–69. DOI: 10.1134/S0012501619050014.Khubutiya M.Sh., Evseev A. K., Kolesnikov V.A., Goldin M.M., Davydov A.D.,Volkov A.G., Stepanov A.A. Measurements of Platinum Electrode Potential in Blood and Blood Plasma and Serum. Russ. J. Electrochem. 2010; 46 (5): 537–541. DOI: 10.1134/S1023193510050071.Khubutiya M.Sh., Evseev A. K., Kolesnikov V.A., Goldin M.M., Davydov A.D.,Volkov A.G., Stepanov A.A. Measurements of Platinum Electrode Potential in Blood and Blood Plasma and Serum. Russ. J. Electrochem. 2010; 46 (5): 537–541. DOI: 10.1134/S1023193510050071.Goldin Michael M., Khubutia M.Sh., Evseev A.K., Goldin Mark M., Pinchuk A.V., Pervakova E.I., Tarabrin Y.A., Hall P.J. Noninvasive Diagnosis of Dysfunctions in Patients After Organ Transplantation by Monitoring the Redox Potential of Blood Serum. Transplantation. 2015; 99 (6): 1288–1292. DOI: 10.1097/tp.0000000000000519. PMID: 25606793.Goldin Michael M., Khubutia M.Sh., Evseev A.K., Goldin Mark M., Pinchuk A.V., Pervakova E.I., Tarabrin Y.A., Hall P.J. Noninvasive Diagnosis of Dysfunctions in Patients After Organ Transplantation by Monitoring the Redox Potential of Blood Serum. Transplantation. 2015; 99 (6): 1288–1292. DOI: 10.1097/tp.0000000000000519. PMID: 25606793.Holland L.L., Brooks J.P. Toward rational fresh frozen plasma transfusion: the effect of plasma transfusion on coagulation test results. Am. J. Clin. Pathol. 2006; 126 (1): 133–139. DOI: 10.1309/NQXHUG7H-ND78-LFFK. PMID: 16753596.Holland L.L., Brooks J.P. Toward rational fresh frozen plasma transfusion: the effect of plasma transfusion on coagulation test results. Am. J. Clin. Pathol. 2006; 126 (1): 133–139. DOI: 10.1309/NQXHUG7H-ND78-LFFK. PMID: 16753596.Shinagare S.A., Angarkar N.N., Desai S.R., Naniwadekar M.R. An audit of fresh frozen plasma usage and effect of fresh frozen plasma on the pre–transfusion international normalized ratio. Asian J. Transfus. Sci. 2010; 4 (2): 128–132. DOI: 10.4103/0973-6247.67024. PMID: 20859514.Shinagare S.A., Angarkar N.N., Desai S.R., Naniwadekar M.R. An audit of fresh frozen plasma usage and effect of fresh frozen plasma on the pre–transfusion international normalized ratio. Asian J. Transfus. Sci. 2010; 4 (2): 128–132. DOI: 10.4103/0973-6247.67024. PMID: 20859514.Shikdar S., Agrawal K., Ghionni N., Hommadov K., Abdollahi S., Micaily I., Green E. The Appropriateness of Fresh Frozen Plasma (FFP) Administration in Three Community Teaching Hospitals. Blood. 2019; 134 (Suppl. 1): 5789. DOI: 10.1182/blood-2019-127693.Shikdar S., Agrawal K., Ghionni N., Hommadov K., Abdollahi S., Micaily I., Green E. The Appropriateness of Fresh Frozen Plasma (FFP) Administration in Three Community Teaching Hospitals. Blood. 2019; 134 (Suppl. 1): 5789. DOI: 10.1182/blood-2019-127693.Abdel-Wahab O.I., Healy B., Dzik W.H. Effect of fresh-frozen plasma transfusion on prothrombin time and bleeding in patients with mild coagulation abnormalities. Transfusion. 2006; 46 (8): 1279–1285. DOI: 10.1111/j.1537-2995.2006.00891.x. PMID: 16934060.Abdel-Wahab O.I., Healy B., Dzik W.H. Effect of fresh-frozen plasma transfusion on prothrombin time and bleeding in patients with mild coagulation abnormalities. Transfusion. 2006; 46 (8): 1279–1285. DOI: 10.1111/j.1537-2995.2006.00891.x. PMID: 16934060.Sezik S., Aksay E., Kýlýç T.Y. The effect of fresh frozen plasma transfusion on international normalized ratio in emergency department patients. J. Emerg. Med. 2014; 47 (5): 596–600. DOI: 10.1016/j.jemermed. 2014.04.042. PMID: 25074780.Sezik S., Aksay E., Kýlýç T.Y. The effect of fresh frozen plasma transfusion on international normalized ratio in emergency department patients. J. Emerg. Med. 2014; 47 (5): 596–600. DOI: 10.1016/j.jemermed. 2014.04.042. PMID: 25074780.

The authors declare that there are no conflicts of interest present.