rmtОбщая реаниматологияGeneral Reanimatology1813-97792411-7110FSBI "SRIGR" RAMS10.15360/1813-9779-2023-3-54-65rmt-2314Research ArticleОБЗОРЫ И КРАТКИЕ СООБЩЕНИЯREVIEWS & SHORT COMMUNICATIONSПрименение фотохимического тромбоза для моделирования ишемического инсульта (обзор)Photochemicallly Induced Thrombosis as a Model of Ischemic StrokeОстроваИ. В.OstrovaI. V.

Ирина Васильевна Острова

Россия, 107031, г. Москва, ул. Петровка, д. 25, стр. 2 

 

 Irina V. Ostrova 

 25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia 

irinaostrova@mail.ruhttps://orcid.org/0000-0003-1780-9829БабкинаА. С.BabkinaA. S.

Анастасия Сергеевна Бабкина, старший научный сотрудник лаборатории патологии клетки при критических состояниях

 Россия, 107031, г. Москва, ул. Петровка, д. 25, стр. 2 

 Anastasia S. Babkina 

 25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia 

ababkina@fnkcrr.ruhttps://orcid.org/0000-0002-1735-592XЛюбомудровМ. А.LyubomudrovM. A.

Максим Алексеевич Любомудров, младший научный сотрудник лаборатории патологии клетки при критических состояниях

 Россия, 107031, г. Москва, ул. Петровка, д. 25, стр. 2 

 Maxim A. Lyubomudrov 

 25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia 

mlyubomudrov@fnkcrr.ruhttps://orcid.org/0000-0003-3318-796XГречкоА. В.GrechkoA. V.

Андрей Вячеславович Гречко, член-корреспондент РАН, директор 

 Россия, 107031, г. Москва, ул. Петровка, д. 25, стр. 2 

 Andrey V. Grechko 

 25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia 

avgrechko@fnkcrr.ruhttps://orcid.org/0000-0002-3165-0378ГолубевА. М.GolubevA. M.

Аркадий Михайлович Голубев, профессор, д.м.н., заведующий лаборатории патологии клетки при критических состояниях

 Россия, 107031, г. Москва, ул. Петровка, д. 25, стр. 2 

 Arkady M. Golubev 

 25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia 

agolubev@fnkcrr.ruФедеральный научно-клинический центр реаниматологии и реабилитологииРоссияFederal Research and Clinical Center of Intensive Care Medicine and RehabilitologyRussian Federation2023240620231935465Copyright © Острова И.В., Бабкина А.С., Любомудров М.А., Гречко А.В., Голубев А.М., 20232023Острова И.В., Бабкина А.С., Любомудров М.А., Гречко А.В., Голубев А.М.Ostrova I.V., Babkina A.S., Lyubomudrov M.A., Grechko A.V., Golubev A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.reanimatology.com/rmt/article/view/2314

Понимание механизмов ишемического повреждения головного мозга имеет важное значение для разработки и совершенствования методов диагностики и лечения ишемического инсульта. Необходимость экспериментальных исследований обусловливает потребность в релевантных моделях фокальных повреждений ткани головного мозга. Одной из наиболее популярных является модель ишемического инсульта, основанная на методе фотохимического тромбоза. Цель обзора — рассмотреть патогенетические основы и прикладное значение метода фотохимического тромбоза при моделировании ишемического инсульта.Материал и методы. Поиск информации проводили с использованием баз данных PubMed и GoogleScholar по ключевым словам «photothrombotic stroke» без языковых ограничений. Из более чем 600 источников для анализа выбрали 74, которые в наибольшей степени соответствовали цели обзора. Из них более 50% были опубликованы в течение последних пяти лет. Критерием исключения источников служило их несоответствие задачам обзора и малая информативность.Результаты. Изложили особенности моделирования фототромботического инсульта, провели анализ преимуществ и недостатков модели, предоставили сведения о ее современных модификациях, методах исследования головного мозга при моделировании инсульта с помощью фототромбоза, обобщили информацию о механизмах развития повреждения мозга в рассматриваемой модели.Заключение. Ряд преимуществ модели фототромботического инсульта: малая инвазивность, высокая воспроизводимость, возможность контроля объема инфаркта мозга и низкая летальность, обусловливают ее активное использование в экспериментальных исследованиях ишемического инсульта. Патологические процессы в головном мозге, моделируемые фотохимическим тромбозом, аналогичны процессам, происходящим при остром нарушении мозгового кровообращения по ишемическому типу. Поэтому данная модель позволяет изучать клеточные и молекулярные механизмы при ишемическом повреждении мозга и может быть полезна для поиска возможностей терапии инсульта.

Better understanding of ischemic brain injury mechanisms is important for the development and improvement of diagnostic and therapeutic modalities for management of ischemic stroke. As experimental studies are on demand, there’s a need for relevant models of focal brain lesions. Photochemically induced thrombosis remains one of the most popular models of ischemic stroke.The purpose of the review is to consider the pathogenesis and applicational relevance of the photochemical thrombosis in ischemic stroke modeling.Material and methods. The information was searched using PubMed and Google Scholar databases and keywords «photothrombotic stroke» without language restrictions. 74 papers out of more than 600 sources were found the most relevant for the purpose of this review and selected for the analysis. Of these, more than 50% have been published in the last five years. The criterion for excluding a source was an inconsistency with the objectives of the review and low information content.Results. We outlined a variety of features in modeling photothrombotic stroke, analyzed the advantages and disadvantages of the model, presented data on current method’s modifications, as well as approaches to evaluation of brain lesions in ischemic stroke induced by photothrombosis, and summarized information about the mechanisms of brain damage induced in this model.Conclusion. Several advantages of the photothrombotic stroke model, such as low invasiveness, high reproducibility, inherent control of brain infarction volume and low mortality, determine its active use in experimental studies of ischemic stroke. Pathological processes in the brain modeled by photochemical thrombosis are similar to the processes occurring in acute ischemic cerebral circulation events. Therefore, this model provides insights into cellular and molecular mechanisms of ischemic brain damage, and can be used for developing novel therapeutic approaches for management of ischemic stroke.

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The authors declare that there are no conflicts of interest present.