Specific Features of Development of the Infectious Process Caused by Cultivable and Non Cultivable Bacteria in the Presence of Experimental Burn Injury
https://doi.org/10.15360/1813-9779-2015-5-15-24
Abstract
Objective: to study a trend in the development of the infectious process caused by cultivable and non cultivable bacteria in Chinchilla rabbits with burn disease.
Materials and methods. The investigators examined 64 rabbits subcutaneously infected with cultivable and non cultivable Pseudomonas aeruginosa and Staphylococcus aureus at a dose of 105 microbial cells in the presence of burn injury in experimental groups and in two similar control groups of animals without thermal injury. Rabbits were exposed to IIIAB degree burn injury under anesthesia. The non cultivable bacteria were obtained by the procedure proposed by L. B. Kozlov et al., by applying a refrigerated heating circulator. The trend in the development of the infectious process was observed during 21 days. Dead animals were dissected; bacterial concentrations were estimated in their viscera and abnormal changes were determined in the histological specimens. The results of the investigations were statistically processed according to STATISTICA 6.0 using the mean arithmetic error (М±m). Differences were statistically evaluated by Student’s t test and the Mann Whitney test. A correlation analysis was made applying the software package Microsoft Excel 97 for IBM PC to compute correlation coefficients and their errors.
Results. The cultivable bacteria in the animals with burn injury induced an infectious process in their viscera to develop sepsis on days 8—12 of the disease with a fatal outcome and the non cultivable bacteria in those with burn disease caused death due to brain injury on days 2—3, with P.aeruginosa isolated from brain tissue.
Conclusion. Both the cultivable and non cultivable bacteria had significant effects in Chinchilla rabbits with burn disease. Non cultivable P.aeruginosa had a tropism for nerve tissue, which was not found in the cul tivable bacteria.
About the Authors
S. P. Sakharov
Tyumen State Medical University, Ministry of Health of the Russian Federation 54, Odesskaya St., Tyumen 625023, Russia
Russian Federation
Russian Federation
L. B. Kozlov
Tyumen State Medical University, Ministry of Health of the Russian Federation 54, Odesskaya St., Tyumen 625023, Russia
Russian Federation
Russian Federation
References
1. Shabanov A.K., Khubutia M.Sh., Bulava G.V., Beloborodova N.V., Kuzovlev A.N., Grebenchikov O.A., Kosolapov D.A., Shpitonkov M.I. Dinamika urovnya prokaltsitonina pri razvitii nozokomialnoi pnev monii u postradavshikh s tyazheloi sochetannoi travmoi v otdelenii reanimatsii. Obshchaya Reanimatologiya. [Time course of changes in the level of procalcitonin in the development of nosocomial pneumonia in victims with severe concomitant injury in an intensive care unit. General Reanimatology]. 2013; 9 (5): 11–17. http://dx.doi.org/ 10.15360/ 1813 9779 2013 5 11. [In Russ.]
2. Yakovlev A.Yu., Gushchina N.N., Niyazmatov A.A., Zaitsev R.M., Golubtsova E.Yu., Ryabikova M.A. Rannyaya otsenka effektivnos ti antibakterialnoi terapii nozokomialnoi pnevmonii putem kolich estvennogo opredeleniya lipopolisakharida. Obshchaya Reanimatologiya. [Early evaluation of the efficiency of antibiotic ther apy for nosocomial pneumonia by quantifying lipopolysaccharide. General Reanimatology]. 2013; 9 (6): 45–52. http://dx.doi.org/ 10.15360/1813 9779 2013 6 45. [In Russ.]
3. Ushakova N.D., Shevchenko A.N., Chetverikov M.V., Zlatnik E.Yu., Zykova T.A. Rezultaty primeneniya selektivnoi adsorbtsii endotoksina pri sep sise u onkologicheskikh bolnykh. Obshchaya Reanimatologiya. [Results of using selective endotoxin adsorption in cancer patients with sepsis. General Reanimatology]. 2014; 10 (6): 32–38. http://dx. doi.org/10.15360/1813 9779 2014 6 32 38. [In Russ.]
4. Shabanov A.K., Bulava G.V., Androsova M.V., Kuzovlev A.N., Kislukhina E.V., Khubutia M.Sh. Rol rannei immunozamestitelnoi terapii v snizhenii chastoty razvitiya nozokomialnoi pnevmonii u postra davshikh s tyazheloi sochetannoi travmoi. Obshchaya Reanimatologiya. [Role of early immune replacement therapy in reduc ing the rate of nosocomial pneumonia in severe polytrauma. General Reanimatology]. 2014; 10 (6): 15–23. http://dx.doi.org/10.15360/ 1813 9779 2014 6 15 23. [In Russ.]
5. Volyansky Yu.L. Nekulturabelnoe sostoyanie asporogennykh bakterii: teoreticheskie aspekty problemy i ee prakticheskaya znachimost. [Nonculturable state of asporogenous bacteria: theoretical aspects of the problem and its practical significance]. Infektsionnye Bolezni (Ukraina). 2004; 1: 5–9. [In Russ.]
6. Sokolenko A.V. Nekultiviruemye formy bakterii: rasprostranenie v prirode, induktory nekultiviruemogo sostoyaniya i reversii. [Non cultured bacte ria: Spread in nature, inducers of a non cultured state and reversion]. Sovremennye Naukoemkie Tekhnologii. 2006; 2: 11–15. [In Russ.]
7. Heim S., Lleo M., Bonato B., Guzman C.A., Canepari P. The viable but nonculturable state and starvation are different stress responses of Enterococcus faecalis, as determined by proteome analysis. J. Bacteriol. 2002; 184 (23): 6739–6745. http://dx.doi.org/10.1128/JB.184.23. 6739–6745.2002. PMID: 12426365
8. Binsztein N., Costagliola M.C., Pichel M., Jurquiza V., Ramírez F.C., Akselman R., Vacchino M., Huq A., Colwell R.R. Viable but noncultur able Vibrio cholerae O1 in the aquatic environment of Argentina. Appl. Environ. Microbiol. 2004; 70 (12): 7481–7486. http://dx.doi.org/ 10.1111/j.1365 2672.2007.03468.x. PMID: 15574951
9. Tafelshtein E.A., Golubinsky E.P., Maramovich A.S., Urbanovich L.Ya., Burenina L.F., Mironova L.V., Sappo S.G., Markov E.Yu., Kapustin Yu.M., Beloborodov Yu.V. Eksperimentalnoe poluchenie nekultiviruemykh form Vibrio cholerae eltor i kharakteristika ikh biologicheskikh svoistv. [Experimental non culturable Vibrio cholerae eltor and its bio logical properties]. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii. 2004; 1: 13–18. PMID: 15024974. [In Russ.]
10. Sokolenko A.V., Menshikova E.A., Aseyeva L.E., Mironova A.V., Titova S.V. Okislitelnyi stress kak prichina obrazovaniya nekultiviruemykh form Vibrio cholerae. [The oxidation stress as a cause of origination of non culturable Vibrio cholerae forms]. Problemy Osobo Opasnykh Infektsii. 2006; 1 (91): 45–49. [In Russ.]
11. Mizunoe Y., Wai S.N., Ishikawa T., Takade A., Yoshida S. Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation. FEMS Microbiol. Lett. 2000; 186 (1): 115–120. http://dx.doi.org/10.1111/j.1574 6968.2000.tb09091.x. PMID: 10779722
12. Shleyeva M.O., Mukamolova G.V., Telkov M.V., Berenzinskaya T.L., Syroeshkin A.V., Biketov S.F., Kaprelyants A.S. Obrazovanie «nekul tiviruemykh» kletok Micobacterium tuberculosis i ikh ozhivlenie. [Formation of nonculturable Mycobacterium tuberculosis and their regeneration]. Mikrobiologiya. 2003; 72 (1): 76–83. PMID: 12698796. [In Russ.]
13. Romanova L.V., Mishankin B.N., Pichurina N.L., Vodopyanov S.O., Sayamov S.R. Nekultiviruemye formy Francisella tularensis. [Noncultivatable forms of Francisella tularensis]. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologi. 2000; 2: 11–15. PMID: 10808564. [In Russ.]
14. Steinert M., Emödy L., Amann R., Hacker J. Resuscitation of viable but non culturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii. Appl. Environ. Microbiol. 1997; 63 (5): 2047–2053. http://dx.doi.org/ 10.1128/AEM.00131 12. PMID: 9143134
15. Chetina E.V. Vliyanie nekotorykh fiziologicheskikh I geneticheskikh faktorov na protsess perekhoda enterotoksigennykh shtammov Escherichia coli v nekultiviruemoe sostoyanie. [The effect of various physiological and genetic factors on the transition process of entero toxigenic strains of Escherichia coli into uncultured state]. Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya. 1997; 1: 8–14. PMID: 9082186. [In Russ.]
16. Makino S.I., Kii T., Asakura H., Shirahata T., Ikeda T., Takeshi K., Itoh K. Does enterohemorrhagic Escherichia coli O157:H7 enter the viable but nonculturable state in salted salmon roe? Appl. Environ. Microbiol. 2000; 66 (12): 5536 5539. http://dx.doi.org/10.1128/AEM.66.12. 5536 5539.2000. PMID: 11097946
17. Alekseyev A.A., Krutikov M.G., Yakovlev V.P. Ozhogovaya infektsiya. Etiologiya, patogenez, diagnostika i lechenie. [Burn infection. Etiology, pathogenesis, diagnosis and treatment]. Moscow: Vuzovskaya Kniga; 2010: 416. [In Russ.]
18. Bairov G.A. Detskaya travmatologiya. [Pediatric traumatology]. Sankt Peterburg: Piter; 2000: 384. [In Russ.]
19. Baranov A.A. Neonatologiya. Natsionalnoe rukovodstvo. [Neonatology. National leadership]. Moscow: GEOTAR Media; 2009: 848. [In Russ.]
20. Ortenberg E.A., Kovaleva N.A., Khristenko Ya.V., Kirushok G.I., Khokhlyavin R.L. Stafilokokkovaya infektsiya v mnogoprofilnom stat sionare: kharakter i rezervy optimizatsii antibakterialnoi terapii. [Staphylococcal infection in multiprofiled hospital: clinical patterns and therapy optimization opportunities]. Meditsinskaya Nauka i Obrazovanie Urala. 2014; 15 (1) (77): 120–124. [In Russ.]
21. Ivanov V.V., Sakharov S.P., Polyakov A.P. Termicheskaya travma u detei – kak sotsialno ekonomicheskaya problema. [Thermal trauma in chil dren is social and economic problem]. Meditsinskaya Nauka i Obrazovanie Urala. 2010; 11 (2–2) (62): 32–33. [In Russ.]
22. Razina A.V. Obshchaya anesteziya krolikov. V kn.: Veterinarnaya med itsina. [General anesthesia of rabbits. In: Veterinary medicine]. Kazan: 2007; 4: 140–142. [In Russ.]
23. Holt J., Krieg N.R. Opredelitel bakterii Berdzhi. [Bergey’s Manual of Systematic Bacteriology]. Moscow: Mir; 1997; 1: 432; 2: 368. [In Russ.]
24. Kozlov L.B., Dits E.V., Efimov V.V., Sannikov A.G., Butkov I.I., Kozlov A.L. Sposob vydeleniya nekultiviruemykh bakterii stafilokokka. Patent RF na izobretenie No2470074. [Procedure for isolation of non cultured Staphylococcus bacteria. RF Patent for invention No.2470074]. [In Russ.]
25. Kozlov L.B., Efimov V.V., Dits E.V., Sannikov A.G., Butkov I.I., Kozlov A.L. Khladotermostat dlya vydeleniya nekultiviruemykh bakterii. Patent RF na izobretenie No125888. [Refrigerated heating circulator for isolation of non cultured bacteria. RF Patent for invention No.125888]. [In Russ.]
26. Kozlov L.B., Sakharov S.P., Dits E.V., Efimov V.V., Vanbender E.L., Butkov I.I., Kozlov A.L., Sennikova E.S., Tusupbekova I.N., Beznosov E.V., Sakharova S.V. Ustroistvo dlya provedeniya eksperimentov na kro likakh. Patent RF na izobretenie No132314. [A device for experiments on rabbits. RF Patent for invention No.132314]. [In Russ.]
27. Khrulev S.E., Vorobyev A.V., Belova A.N. Porazheniya golovnogo mozga pri termicheskoi travme: osobennosti klinicheskoi kartiny i factory riska. [Brain lesions in thermal injury: clinical features and risk factors]. Travmatologiya i Ortopediya Rossii. 2007; 3 (45): 31–35. [In Russ.]
28. Khrulev S.E. Uroven neiron spetsificheskoi enolazy v syvorotke krovi bolnykh s ozhogovoi travmoi. [The level of neuron specific enolase in the serum of patients with burn injury]. Sovremennye Naukoemkie Tekhnologii. 2009; 6: 53–54. [In Russ.]
29. Khrulev S.E. Kliniko kompyuterno tomograficheskie korrelyatsii entse falopatii u detei s ozhogovoi travmoi. [Clinical computed tomographic cor relations of encephalopathy in children with burn injury]. Meditsinskaya Vizualizatsiya. Spetsialnyi Vypusk. 2009: 457–458. [In Russ.]
Review
For citations:
Sakharov S.P., Kozlov L.B. Specific Features of Development of the Infectious Process Caused by Cultivable and Non Cultivable Bacteria in the Presence of Experimental Burn Injury. General Reanimatology. 2015;11(5):15-24. https://doi.org/10.15360/1813-9779-2015-5-15-24
Views:
1332
Email this article 