Homogeneous Deformation of Native Erythrocytes During Long-Term Storage
https://doi.org/10.15360/1813-9779-2019-5-4-10
Abstract
Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.
Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.
Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.
Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus.
About the Authors
E. A. Manchenko
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology,
I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia
8 Trubetskaya Str., Bldg. 2, 119991 Moscow, Russia
E. K. Kozlova
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology,
I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia
8 Trubetskaya Str., Bldg. 2, 119991 Moscow, Russia
V. A. Sergunova
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Russian Federation
Russian Federation
25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia
A. M. Chernysh
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology,
I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
25 Petrovka Str., Bldg. 2, 107031 Moscow, Russia
8 Trubetskaya Str., Bldg. 2, 119991 Moscow, Russia
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Review
For citations:
Manchenko E.A., Kozlova E.K., Sergunova V.A., Chernysh A.M. Homogeneous Deformation of Native Erythrocytes During Long-Term Storage. General Reanimatology. 2019;15(5):4-10. https://doi.org/10.15360/1813-9779-2019-5-4-10
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