Atomic Force Microscopy of the Structure of Red Blood Cell Membranes in Acute Blood Loss and Reinfusion
https://doi.org/10.15360/1813-9779-2009-5-5
Abstract
Objective: to examine the surface heterogeneities of red blood cell membranes after acute massive blood loss and blood reinfusion, by using atomic force microscopy (AFM). Materials and methods. Experiments were carried out under nembu-tal anesthesia on male rats. Hypovolemic hypotension was induced during 60 minutes, followed by blood reinfusion. The experimental phases were as follows: control before blood loss; 5 minutes after its onset; 1 hour following hypotension; and 1 and 3 hours after reinfusion. Membrane surface images were obtained by AFM in the constant scanning mode. Spatial surface spectral transformation was used. Results. The nano-surface parameters were shown to be intrinsic characteristics of membranes. The greatest changes occurred at the early stages of transient processes: blood loss and reinfusion. Conclusion. The application of AFM permitted the authors to trace the time course of changes in the red blood cell membrane surfaces during acute total blood loss and further blood reinfusion to tolerances of fractions of nanometer. Key words: blood loss, reinfusion, nanostructure, atomic force microscopy.
About the Authors
V. V. MorozA. M. Chernysh
Ye. K. Kozlova
A. K. Kirsanova
I. S, Novoderzhkina
V. V. Aleksandrin
P. Yu. Borshchegovskaya
U. A. Bliznyuk
R. M. Rysayeva
References
1. Радаев С. М., Остапченко Д. А., Розенберг Ю. М. и соавт.
2. Betz T., Bakowsky U., Muller M. R. et al.Conformational change of membrane proteins leads to shape changes of red blood cells. Bioelectrochemistry 2007; 70 (1): 122—126.
3. Girasole M., Pompeo G., Cricenti A. et al.Roughness of the plasma membrane as an independent morphological parameter to study RBCs: A quantitative atomic force microscopy investigation. Biochim. Biophys. Acta 2007; 1768 (5): 1268—1276.
4. Yamashina S., Katsumata O.Structural analysis of red blood cell membrane with an atomic force microscope. J. Electron. Microsc. 2000; 49 (3): 445—451.
5. Guha T., Bhattacharyya K., Bhar R. et al.Holes on erythrocyte membrane and its roughness contour imaged by atomic force
6. microscopy and lateral force microscopy. Curr. Science 2002; 83 (6): 693—694.
7. Tekle E., Astumian R. D., Friauf W. A. et al.Chock asymmetric pore distribution and loss of membrane lipid in electroporated DOPC vesicles. Biophys. J. 2001; 81 (2): 960—968.
8. Кожура В. Л., Кирсанова А. К., Березина Т. Л. и соавт.Влияние низкоэнергетического лазерного излучения на клетки крови при геморадическом шоке. Тр. Института общей реаниматологии РАМН. т. 2. 2001. 177—182.
9. Фёдорова М. З., Павлов Н. А., Зубарев Е. В. и соавт.Использование атомной силовой микроскопии для оценки морфометрических показателей клеток крови. Биофизика 2008; 53 (6): 1014—101
10. Strasser S., Zink A., Kada G. et al.Age determination of blood spots in forensic medicine by force spectroscopy Forensic Sci. Int. 2007; 170 (1): 8—14.
11. Xiao Yu, Feng Hua, Ping Hui, Jia Zuan.Studies on the effects of epimedium extract on erythrocytes. Chinese Chem. Letters 2006; 17 (8): 1105—1108.
Review
For citations:
Moroz V.V., Chernysh A.M., Kozlova Ye.K., Kirsanova A.K., Novoderzhkina I.S., Aleksandrin V.V., Borshchegovskaya P.Yu., Bliznyuk U.A., Rysayeva R.M. Atomic Force Microscopy of the Structure of Red Blood Cell Membranes in Acute Blood Loss and Reinfusion . General Reanimatology. 2009;5(5):5. (In Russ.) https://doi.org/10.15360/1813-9779-2009-5-5
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