Detection of Occult Erythrocytic Membrane Damages upon Pharmacological Exposures
https://doi.org/10.15360/1813-9779-2007-4-102-105
Abstract
Blood administration of pharmaceuticals may cause occult effects of these agents on erythrocytic membranes. These effects may damage and cause additional membrane defects, but may strengthen. The type and degree of the effects of an agent were detected by calibrated irreversible electroporation with a pulsed electric field (PEF). The paper considers the erythrocytic membranous effects of a wide concentration range of agents used in anesthesiology, such as esmerone, tracrium, and mar-caine-adrenaline. Under the action of PEF and esmerone at the normal concentration N, the rate of erythrocytic hemolysis increased by several times as compared with the control. The similar effect also occurred when esmerone was added at the concentration C=10N. Tracrium exerted a fixing effect on erythrocytic membranes. Upon a combined exposure to PEF and tracrium in the normal concentration C=N; erythrocytic hemolysis was slow. So was with the concentration C=10N. The rate of hemolysis of the red blood cells subjected to a combined action of marcaine adrenaline at the normal concentration C=N and even at the concentration C=10N and PEF was comparable with the hemolytic rate of the reference suspension.
About the Authors
P. Yu. Alekseyeva
Faculty of Physics, M.V. Lomonosov Moscow State University
V. V Moroz
Research Institute of General Reanimatology, Russian Academy of Medical Sciences
U. A. Bliznyuk
Faculty of Physics, M.V. Lomonosov Moscow State University
V. M. Yelagina
Faculty of Physics, M.V. Lomonosov Moscow State University
G. R. Kaziyev
Research Institute of General Reanimatology, Russian Academy of Medical Sciences
V. Yu. Vasilyev
Research Institute of General Reanimatology, Russian Academy of Medical Sciences
A. M. Chernysh
I. M. Sechenov Moscow Medical Academy
M. S. Bogushevich
Research Institute of General Reanimatology, Russian Academy of Medical Sciences
References
1. Козлова Е. К., Черняев А. П., Черныш А. М., Алексеева П. Ю. Электропорация — эффективный метод экспресс-диагностики повреждений биологических мембран в результате воздействия физико-химических факторов на эритроциты. Препринт НИИЯФ МГУ — 2005-/773.
2. Gehl J. Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research. Acta Physiol. Scand. 2003; 177: 437—447.
3. Козлова Е. К., Черняев А. П., Черныш А. М. и др. Модель кинетики гемолиза эритроцитов при действии пучка электронов и импульсного электрического поля. Вестн. Моск. ун-та. Сер. 3. Физика. Астрономия 2004; 2: 19—22.
4. Abidor I. G., Li L. H., Hui S. W. et al. Studies of cell pellets: II. Osmotic properties, Electroporation, and related phenomena: membrane interactions. Biophys. J. 1994; 67 (1): 427—435.
5. Genco I., Gliozzi A., Relini A. et al. Electroporation in symmetric and asymmetric membranes Biochim. Biophys. Acta 1993; 1149 (1): 1—18.
6. Kinosita K., Tsong T. Y. Hemolysis of human erythrocytes by transient electric field. Proc.Natl. Sci. 1977; 74(5): 1923—1927.
Review
For citations:
Alekseyeva P.Yu., Moroz V.V., Bliznyuk U.A., Yelagina V.M., Kaziyev G.R., Vasilyev V.Yu., Chernysh A.M., Bogushevich M.S. Detection of Occult Erythrocytic Membrane Damages upon Pharmacological Exposures. General Reanimatology. 2007;3(4):102-105. (In Russ.) https://doi.org/10.15360/1813-9779-2007-4-102-105
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