Stress Induced Platelet Dysfunction in Rats with Folic Acid-Deficient Hyperhomocysteinemia
https://doi.org/10.15360/1813-9779-2006-6-61-65
Abstract
A rat model of experimental hyperhomocysteinemia (HHC) caused by chronic folic acid deficiency was used to study the impact of swimming stress on the values of agonist-induced platelet aggregation and ATP release. At week 8 of HHC development, platelet aggregability and ATP levels in response to test inductors (ADP, thrombin, and collagen) moderately increased. After stress, the rate of platelet aggregation showed a more increase in rats with HHC; however, the increment was less pronounced than in the control group. The similar changes was observed in ATP, the release of which from dense platelet granules decreased in the experimental animals exposed to stress changes as compared to the controls. The experimental findings suggest that the stress potentiates platelet dysfunction in HHC.
About the Authors
S. V. Alisievitch
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
A. G. Dubichev
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
A. A. Levina
Hematology Research Center, Russian Academy of Medical Sciences
G. A. Nazarova
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
N. N. Zolotov
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
V. V, Krzhechkovskaya
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
N. N. Pavlova
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
E. P. Romanova
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
I. A. Rudko
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
K. A. Cherkasova
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
A. A. Kubatiev
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences
References
1. Wang H. X., Mittleman M. A., OrthZGomer K. Influence of social support on progression of coronary artery disease in women. Soc. Sci. Med. 2005; 60 (3): 599—607.
2. OrthZGomer K., Wamala S. P., Horsten M. et al. Marital stress worsens prognosis in women with coronary heart disease: The Stockholm Female Coronary Risk Study. J. A. M. A. 2000; 284: 3008—3014.
3. Nemeroff C. B., Musselman D. L., Evans D. L. Depression and cardiac disease. Depress. Anxiety 1998; 8 (Suppl. 1): 71—79.
4. Rozanski A., Blumenthal J. A., Kaplan J. Impact of psychological factors on the pathogenesis of cardiovascular disease and implications for therapy. Circulation 2000; 25: 177—178.
5. Gidron Y., Gilutz H., Berger R., Huleihel M. Molecular and cellular interface between behavior and acute coronary syndromes. Cardiovasc. Res. 2002; 56 (1): 15—21.
6. von Kanel R., Mills P. J., Fainman C., Dimsdale J. E. Effects of psychological stress and psychiatric disorders on blood coagulation and fibrinolysis: a biobehavioral pathway to coronary artery disease. Psychosom. Med. 2001; 63 (4): 531—544.
7. Kario K., Matsuo T., Kobayashi H. et al. Earthquake-induced potentiation of acute risk factors in hypertensive elderly patients: possible triggering of cardiovascular events after a major earthquake. J. Am. Coll. Cardiol. 1997; 29 (5): 926—933.
8. Araki A., Sako Y. Determination of free and total homocysteine in human plasma by high-performance liquid chromatography with fluorescence detection. J. Chromatogr. Biomed. Appl. 1987; 422—443.
9. Кудрин В. С., Мирошниченко И. И., Раевский К. С. Различия в механизмах ауторецепторной регуляции биосинтеза и высвобождения дофамина в подкорковых структурах мозга крыс. Нейрохимия 1988; 1: 3—8.
10. Левина А. А., Мелик-Нубаров Н. С., Казюкова Т. В. и др. Разработка иммуноферментного метода для определения витамина В12 и фолиевой кислоты. В кн.: Биомедприбор-2000: Тез. докл. конф. М.; 2000.
11. Cardinal D. C., Flower R. J. The electronic aggregometer: A novel device for assessing platelet behavior in blood. J. Pharmacol. Meth. 1980; 3: 135—158.
12. Born G. V. Aggregation of blood platelets by ADP and its reversal. Nature 1962; 194: 927—929.
13. O'Brien J. R. Platelet aggregation. Part I Some effect of the adenosine phosphates, thrombin, and cocaine upon platelet adehesiveness. J. Clin. Path. 1962; 15 (5): 446—452.
14. O'Brien J. R. Platelet aggregation. Part II Some results from a new method of study. J. Clin. Path. 1962; 15 (5): 452—455.
15. Островский Ю. М. (ред. ) Экспериментальная витаминология. Минск.: Наука и техника; 1979.
16. Langley-Evans S. C., Gardner D. S., Jackson A. A. Association of disproportionate growth of fetal rats in late gestation with raised systolic blood pressure in later life. J. Reprod. Fertil. 1996; 106: 307—312.
17. Akesson B., Fehling C., Jagerstad M., Stenram U. Effect of experimental folate deficiency on lipid metabolism in liver and brain. Br. J. Nutr. 1982; 47: 505.
18. Zhu B. T. On the mechanism of homocysteine pathophysiology and pathogenesis: a unknown hypothesis. Histol. Histopathol. 2002; 17 (4): 1283—1291.
19. Ungvari Z., Sarkadi-Nagy E., Bagi Z. et al. Simultaneously increased TxA2 activity in isolated arterioles and platelets of rats with hyperhomocysteinemia. Arterioscler. Thromb. Vasc. Biol. 2000; 20 (5): 1203— 1208.
20. Durand P., Prost M., Blache D. Folic acid deficiency enhances oral contraceptive-induced platelet hyperactivity. Arterioscler. Thromb. Vasc. Biol. 1997; 17: 1939—1946.
21. Wilson K. M., Lentz S. R. Mechanisms of the atherogenic effects of elevated homocysteine in experimental models. Semin. Vasc. Med. 2005; 5 (2): 163—171.
22. Maayani S., Schwarz T., Craddock-Royal B., Tagliente T. M. Activation of the alpha(2A)-adrenoceptor mediates deceleration of the deaggregation component of the response to ADP or 5-HT in human platelets in vitro. Platelets 2001; 12 (6): 359—375.
23. Yun-Choi H. S., Pyo M. K., Chang K. C., Lee D. H. The effects of higenamine on LPS-induced experimental disseminated intravascular coagulation (DIC) in rats. Planta Med. 2002; 68 (4): 326—329.
24. Harpel P. C., Zhang X., Borth W. Homocysteine and hemostasis: pathogenic mechanisms predisposing to thrombosis. J. Nutr. 1996; 126 (4 Suppl): 1285—1291.
25. Signorello M. G., Pascale R., Leoncini G. Effect of homocysteine on arachidonic acid release in human platelets. Eur. J. Clin. Invest. 2002; 32 (4): 279—284.
26. Birk A. V., Endri L., Robertson H. D. et al. Interaction between ATP and catecholamines in stimulation of platelet aggregation. Am. J. Physiol. Heart Circ. 2003; 284 (2): 619—625.
Review
For citations:
Alisievitch S.V., Dubichev A.G., Levina A.A., Nazarova G.A., Zolotov N.N., Krzhechkovskaya V.V., Pavlova N.N., Romanova E.P., Rudko I.A., Cherkasova K.A., Kubatiev A.A. Stress Induced Platelet Dysfunction in Rats with Folic Acid-Deficient Hyperhomocysteinemia. General Reanimatology. 2006;2(6):61-65. (In Russ.) https://doi.org/10.15360/1813-9779-2006-6-61-65
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