Blood Rheological Properties in Blood Loss (An experimental study)
https://doi.org/10.15360/1813-9779-2014-5-27-32
Abstract
Blood loss is a complex pathological process with the mechanism that has not been fully unstudied. Blood rheology is a particular link that is involved in blood losses. Objective: to perform a comprehensive study of blood rheological proper ties, such as erythrocyte aggregability, erythrocyte deformability, plasma viscosity, and packed cell volume, in different degrees of experimental blood loss.
Materials and methods. Blood loss of different stages was simulated in anesthetized animals. The Georgian technique, membrane filtration, a capillary method, and a standard centrifugation method were used to study blood rheological properties. The data were analyzed applying the statistical programs Origin 4.1 (Microcat.Software.Inc) and Microsoft Excel.
Results. In stages 1, 2, and 3 blood losses, erythrocyte aggregability was significantly increased by 10, 25, and 44% and erythrocyte deformability was decreased by 12, 13, and 15%, respectively, as compared to the controls. The delta viscosity was insignificant (both in the subgroups in different blood loss degrees
versus the control group); the packed cell volume in blood loss was increased insignificantly as compared to the control.
Conclusion. Comprehensive assessment of the degree of blood loss requires a minimum set of blood rheological indices, such as erythrocyte aggregability and deformability, blood viscosity, and packed cell volume. The basis for monitoring the rheological indices of massive blood loss is the specific features of erythrocyte aggregation.
About the Authors
M. M. Mantskava
I. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
Russian Federation
14, L. Gotua, Tbilisi, Georgia
Russian Federation
14, L. Gotua, Tbilisi, Georgia
N. G. Momtselidze
I. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
Russian Federation
14, L. Gotua, Tbilisi, Georgia
Russian Federation
14, L. Gotua, Tbilisi, Georgia
L. Sh. Davlianidze
I. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
Russian Federation
14, L. Gotua, Tbilisi, Georgia
Russian Federation
14, L. Gotua, Tbilisi, Georgia
References
1. Likhovetskaya Z.M., Prigozhina T.A., Gorbunova N.A. Gemoreologicheskie narusheniya pri shoke razlichnoi etiologii. [Hemorheologic disorders in shock of diverse etiology]. Byulleten Eksperimentalnoi Biologii i Meditsiny. 1988: 106 (10): 426—428. PMID: 3191229. [In Russ.]
2. Moroz D.V., Shapovalova N.V. Vliyanie giperbaricheskoi oksigenatsii i reologicheskie svoistva krovi pri shoke. [Impact of hyperbaric oxygenation on hemostasis and blood rheological properties in shock]. Byulleten Giperbaricheskoi Biologii i Meditsiny. 1997; 1—2: 31—37. [In Russ.]
3. Moroz D.V., Shapovalova N.V. Sostoyanie gemostaza i reologicheskikh svoistv krovi pri lechenii shoka. Materily nauchnoi konferentsii «Aktualnye voprosy abdominalnoi khirurgii». [Hemostasis and blood rheological properties in the treatment of shock. Proceedings of the Scientific Conference on the Topical Aspects of Abdominal Surgery]. Kursk; 1997. [In Russ.]
4. Sordia T., Tatarishvili J., Varazashvili M., Mchedlishvili G. Hemorheological disorders in the microcirculation following hemor rhage. Clin. Hemorheol. Microcirc. 2004; 30 (3—4): 461—462. PMID: 15258387
5. Stoltz J., Donner M. Erythrocyte aggregation: experimental approaches and clinical implications. Int. Angiol. 1987; 6 (2): 193—201. PMID: 3323355
6. Mantskava M.M., Momtselidze N.G., Davlianidze L.Sh. Novaya klassifikatsiya stadii gemorragicheskogo shoka, osnovannaya na izmenenii agregatsii eritrotsitov. Materily Pervoi konferentsii Rossiiskogo Natsionalnogo obshchestva po izucheniyu shoka. 3 oktyabrya 2013 g. [New hemorrhagic stages classification based on platelet aggregation changes. Proceedings of the First Conference of the Russian NationalSociety of Shock Study. 3 October 2013]. Moscow; 2013: 42—43. [In Russ.]
7. Kovalenko N.Ya., Matsievsky D.D. Serdechnososudistaya sistema u krys s razlichnoi ustoichivostyu k ostroi krovopotere. [Cardiovascular system in rats with varying resistance to acute hemorrhage]. Patologicheskaya Fiziologiya i Eksperimentalnaya Terapiya. 1998; 2: 32—36. PMID: 9633197. [In Russ.]
8. Baskurt O., Neu B., Meiselman H. Red blood cell aggregation. CRS Press; 2012: 1—26.
9. Mchedlishvili G., Beritashvili N., Lominadze D., Tsinamdzgvrishvili B. Technique for direct and quantitative evaluation of erythrocyte aggre gability on blood samples. Biorheology. 1993; 30 (2): 153—161.PMID: 8400153
10. Reid H.L., Barnes A.J., Lock P.J., Dormandy J.A., Dormandy T.L. A simple method for measuring erythrocyte deformability. J. Clin. Pathol. 1976; 29 (9): 855—858. PMID: 977787
11. L°c°tusøu D., C°runtu I.D., Rusu V. Study on erythrocyte aggregation using computerized image analysis methods. Rev. Med. Chir. Soc. Med. Nat. Iasi. 2013; 117 (3): 801—805. PMID: 24502054
12. Mchedlishvili G.I. Mikrotsirkulyatsiya krovi. [Blood microcirculation]. Leningrad: Nauka; 989: 295. [In Russ.]
13. Myuller M., Algover M., Shnaider R., Villinegger Kh. Rukovodstvo po vnutrennemu osteosintezu. [A manual of internal osteosynthesis]. Moscow; 1996. [In Russ.]
14. Ryabov G.A. Anesteziologiya i reanimatologiya. [Anesthesiology and Reanimatology]. Moscow: Meditsina Publishers; 1983. [In Russ.]
15. Bishop J.J., Nance P.R., Popel A.S., Intaglietta M., Johnson P.C. Effect of erythrocyte aggregation on velocity profiles in venules. Am. J. Physiol. Heart Circ. Physiol. 2001; 280 (1): H222—H236. PMID: 11123237
Review
For citations:
Mantskava M.M., Momtselidze N.G., Davlianidze L.Sh. Blood Rheological Properties in Blood Loss (An experimental study). General Reanimatology. 2014;10(5):27-32. https://doi.org/10.15360/1813-9779-2014-5-27-32
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