Effect of Perftoran on Membrane Nanostructure of Discocyte and Stomatocyte after Acute Blood Loss

The purpose of the study: to evaluate the effect of Perftoran (PF) on the membrane nanostructure of discocytes and stomatocytes after acute blood loss. Materials and methods. The study was performed in white male rats. Nembutal was administered intraperitoneally at a dose of 25 mg/kg. The study model was as follows: acute blood loss followed by hypotension for 60 min with the BP maintained at the level of 40 mm Hg with subsequent reinfusion of lost blood. Blood samples were collected from the tail artery before the blood loss and 2 hours after reinfusion. PF was administered intraarterially at a dose 3 ml/kg of body mass immediately after the blood loss replacement. Animals who did not receive Perftoran served as a reference group. The RBC membrane structure was studied using the Femtoscan atomic force microscope at ranges with different spatial periods. The ranges were selected using the Fourier transform and spectral windowing. Periods (T) and heights (h) of RBC surface corrugation were calculated for each spectral window according to the isolated profiles of the membrane surface. Results. At a baseline; discocytes and stomatocytes exhibited different structural characteristics of RBC membrane at a spatial range of 600—1200 nm in the form of a higher period of vibration of stomatocytes vs. discocytes. Hypovolemia and subsequent reinfusion of autologous blood affected studied parameters by increasing the period of spatial oscillation both in discocytes and stomatocytes as compared to the initial state. Administration of PF significantly decreased the period of spatial oscillation of the cell membrane both in discocytes and stomatocytes as compared to animal RBCs who underwent hypovolemia and reinfusion without PF administration and baseline parameters. Conclusion. The period of vibration within the range of 600"1200 nm reflecting the state of the bilayer of RBC membrane is not related to the discocyte transformation into the stomatocyte. Data suggest that the PF-induced shortage in vibration within this range was associated with the increased ability of a RBC to deform.

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