Новые методы лечения при сепсисе: модели на животных «не работают» (обзор)
Аннотация
Сепсис, как и многие патологические инфекционные процессы, в основном изучается in vivo с использованием моделей на мышах. За последние 30 лет подобные исследования привели к значимым достижениям в понимании патофизиопатологии сепсиса. Однако, к сожалению, ни одно из них не привело к каким-либо «открытиям» в терапии пациентов. В настоящем обзоре мы ставим под сомнение актуальность используемых экспериментальных моделей, перечислим некоторые из редко принимаемых во внимание аспектов и обсудим пути выхода из создавшегося тупика. Ключевые слова: модели на животных; лечение сепсиса
Текст является переводом статьи Cavaillon J. M. Nouvelles therapies du sepsis: l'echec des modeles animaux, Bull. Assoc. Anc. El. Inst. Pasteur, 2017, 59, 230, 58—60. Перевод с французского выполнен фирмой «Академперевод», Москва, Россия.
Список литературы
1. Baker C.C., Chaudry I.H., Gaines H.O., Baue A.E. Evaluation of factors affecting mortality rate after sepsis in a murine cecal ligation and puncture model. Surgery. 1983; 94 (2): 331-335. PMID: 6879447
2. Chaudry I.H., Hirasawa H., Baue A.E. Impairment of reticuloendothelial system function with sepsis and its improvement with ATP-MgCl2 plus glucose administration. Adv. Shock Res. 1979; 2: 153-162. PMID: 262800
3. Dejager L., Pinheiro I., Dejonckheere E., Libert C. Cecal ligation and puncture: the gold standard model for polymicrobial sepsis? Trends Microbiol. 2011; 19 (4): 198-208. DOI: 1016/j.tim.2011.01.001. PMID: 21296575
4. Davaine C. Recherches sur les infusoires du sang dans la maladie connue sous le nom de sang de rate. C.R. Acad. Sci. 1863; 57: 220-223, 351-353.
5. Warren H.S., Fitting C., Hoff E., Adib-Conquy M., Beasley-Topliffe L., Tesini B., Liang X., Valentine C., Hellman J., Hayden D., Cavaillon J.M. Resilience to bacterial infection: difference between species could be due to proteins in serum. J. Infect. Dis. 2010; 201 (2): 223-232. DOI: 10.1086/649557. PMID: 20001600
6. Hajjar A.M., Ernst R.K., Tsai J.H., Wilson C.B., Miller S.I. Human toll-like receptor 4 recognizes host-specific LPS modifications. Nat. Immunol. 2002; 3 (4): 354-359. DOI: 10.1038/ni777. PMID: 11912497
7. Mora-Rillo M., Fernández-Romero N., Navarro-San Francisco C., DíezSebastián J., Romero-Gómez M.P., Fernández F.A., López J.R.A., Mingorance J. Impact of virulence genes on sepsis severity and survival in Escherichia coli bacteremia. Virulence. 2015; 6 (1): 93-100. DOI: 10.4161/21505594.2014.991234. PMID: 25654604
8. Seboxa T., Amogne W., Abebe W., Tsegaye T., Azazh A., Hailu W., Fufa K., Grude N., Henriksen T.H. High mortality from blood stream infection in Addis Ababa, Ethiopia, is due to antimicrobial resistance. PLoS One. 2015; 10 (2): e0144944. DOI: 10.1371/journal.pone.0144944. PMID: 26670718
9. Nguyen Q.T., Nguyen T.H., Ju S.A., Lee Y.S., Han S.H., Lee S.C., Kwon B.S., Yu R., Kim G.Y., Lee B.J., Kim B.S. CD137 expressed on neutrophils plays dual roles in antibacterial responses against Gram-positive and Gramnegative bacterial infections. Infect. Immun. 2013; 81 (6): 2168-2177. DOI: 10.1128/IAI.00115-13. PMID: 23545301
10. Blanchet C., Jouvion G., Fitting C., Cavaillon J.M., Adib-Conquy M. Protective or deleterious role of scavenger receptors SR-A and CD36 on host resistance to Staphylococcus aureus depends on the site of infection. PLoS One. 2014; 9 (1): e87927. DOI: 10.1371/journal.pone.0087927. PMID: 24498223
11. Deng M., Scott M.J., Loughran P., Gibson G., Sodhi C., Watkins S., Hackam D., Billiar T.R. Lipopolysaccharide clearance, bacterial clearance, and systemic inflammatory responses are regulated by cell type-specific functions of TLR4 during sepsis. J. Immunol. 2013; 190 (10): 5152-5160. DOI: 10.4049/jimmunol.1300496. PMID: 23562812
12. Metzger D.W., Salmon S.L., Kirimanjeswara G. Differing effects of interleukin-10 on cutaneous and pulmonary Francisella tularensis live vaccine strain infection. Infect. Immun. 2013; 81 (6): 2022-2027. DOI: 10.1128/IAI.00024-13. PMID: 23529615
13. von Köckritz-Blickwede M., Rohde M., Oehmcke S., Miller L.S., Cheung A.L., Herwald H., Foster S., Medina E. Immunological mechanisms underlying the genetic predisposition to severe Staphylococcus aureus infection in the mouse model. Am. J. Pathol. 2008; 173 (6): 1657-1668. DOI: 10.2353/ajpath.2008.080337. PMID: 18974303
14. Gingles N.A., Alexander J.E., Kadioglu A., Andrew P.W., Kerr A., Mitchell T.J., Hopes E., Denny P., Brown S., Jones H.B., Little S., Booth G.C., McPheat W.L. Role of genetic resistance in invasive pneumococcal infection: identification and study of susceptibility and resistance in inbred mouse strains. Infect. Immun. 2001; 69 (1): 426-434. DOI: 10.1128/IAI.69.1.426-434.2001. PMID: 11119534
15. De Albuquerque N., Baig E., Ma X., Zhang J., He W., Rowe A., Habal M., Liu M., Shalev I., Downey G.P., Gorczynski R., Butany J., Leibowitz J., Weiss S.R., McGilvray I.D., Phillips M.J., Fish E.N., Levy G.A. Murine hepatitis virus strain 1 produces a clinically relevant model of severe acute respiratory syndrome in A/J mice. J. Virol. 2006; 80 (21): 10382-10394. DOI: 10.1128/JVI.00747-06. PMID: 17041219
16. Watanabe H., Numata K., Ito T., Takagi K., Matsukawa A. Innate immune response in Th1and Th2-dominant mouse strains. Shock. 2004; 22 (5): 460-466. DOI: 10.1097/01.shk.0000142249.08135.e9. PMID: 15489639
17. Hultgren O., Kopf M., Tarkowski A. Outcome of Staphylococcus aureustriggered sepsis and arthritis in IL-4-deficient mice depends on the genetic background of the host. Eur. J. Immunol. 1999; 29 (8): 2400-2405. DOI: 10.1002/(SICI)1521-4141(199908)29:083.0.CO;2-E. PMID: 10458752
18. Wells C.A., Ravasi T., Faulkner G.J., Carninci P., Okazaki Y., Hayashizaki Y., Sweet M., Wainwright B.J., Hume D.A. Genetic control of the innate immune response. BMC Immunol. 2003; 4: 5-22. DOI: 10.1186/14712172-4-5. PMID: 12826024
19. Klein S.L., Flanagan K.L. Sex differences in immune responses. Nat. Rev. Immunol. 2016; 16 (10): 626-638. DOI: 10.1038/nri.2016.90. PMID: 27546235
20. Pasche B., Kalaydjiev S., Franz T.J., Kremmer E., Gailus-Durner V., Fuchs H., Hrabé de Angelis M., Lengeling A., Busch D.H. Sex-dependent susceptibility to Listeria monocytogenes infection is mediated by differential interleukin-10 production. Infect. Immun. 2005; 73 (9): 5952-5960. DOI: 10.1128/IAI.73.9.5952-5960.2005. PMID: 16113316
21. Zellweger R., Wichmann M.W., Ayala A., Stein S., DeMaso C.M., Chaudry I.H. Females in proestrus state maintain splenic immune functions and tolerate sepsis better than males. Crit. Care Med. 1997; 25 (1): 106-110. DOI: 10.1097/00003246-199701000-00021. PMID: 8989185
22. Harizi H., Homo-Delarche F., Amrani A., Coulaud J., Mormède P. Marked genetic differences in the regulation of blood glucose under immune and restraint stress in mice reveals a wide range of corticosensitivity. J. Neuroimmunol. 2007; 189 (1-2): 59-68. DOI: 10.1016/j.jneuroim.2007.06.019. PMID: 17658621
23. Tateda K., Matsumoto T., Miyazaki S., Yamaguchi K. Lipopolysaccharideinduced lethality and cytokine production in aged mice. Infect. Immun. 1996; 64 (3): 769-774. PMID: 8641780
24. Saito H., Sherwood E.R., Varma T.K., Evers B.M. Effects of aging on mortality, hypothermia, and cytokine induction in mice with endotoxemia or sepsis. Mech. Ageing Dev. 2003; 124 (10-12): 1047-1058. DOI: 10.1016/j.mad.2003.08.002. PMID: 14659593
25. Starr M.E., Evers B.M., Saito H. Age-associated increase in cytokine production during systemic inflammation: adipose tissue as a major source of IL-6. J. Gerontol. A Biol. Sci. Med. Sci. 2009; 64 (7): 723-730. DOI: 10.1093/gerona/glp046. PMID: 19377014 2
26. Mirsoian A., Bouchlaka M.N., Sckisel G.D., Chen M., Pai C.C., Maverakis E., Spencer R.G., Fishbein K.W., Siddiqui S., Monjazeb A.M., Martin B., Maudsley S., Hesdorffer C., Ferrucci L., Longo D.L., Blazar B.R., Wiltrout R.H., Taub D.D., Murphy W.J. Adiposity induces lethal cytokine storm after systemic administration of stimulatory immunotherapy regimens in aged mice. J. Exp. Med. 2014; 211 (12): 2373-2383. DOI: 10.1084/jem.20140116. PMID: 25366964
27. Lambeck S., Weber M., Gonnert F.A., Mrowka R., Bauer M. Comparison of sepsis-induced transcriptomic changes in a murine model to clinical blood samples identifies common response patterns. Front. Microbiol. 2012; 3: 284. DOI: 10.3389/fmicb.2012.00284. PMID: 23024636
28. Scheiermann C., Kunisaki Y., Lucas D., Chow A., Jang J.E., Zhang D., Hashimoto D., Merad M., Frenette P.S. Adrenergic nerves govern circadian leukocyte recruitment to tissues. Immunity. 2012; 37 (2): 290-301. DOI: 10.1016/j.immuni.2012.05.021. PMID: 22863835
29. Castanon-Cervantes O., Wu M., Ehlen J.C., Paul K., Gamble K.L., Johnson R.L., Besing R.C., Menaker M., Gewirtz A.T., Davidson A.J. Dysregulation of inflammatory responses by chronic circadian disruption. J. Immunol. 2010; 185 (10): 5796-5805. DOI: 10.4049/jimmunol.1001026. PMID: 20944004
30. Martin B., Ji S., Maudsley S., Mattson M.P. «Control» laboratory rodents are metabolically morbid: why it matters. Proc. Natl. Acad. Sci. U S A. 2010; 107 (14): 6127-6133. DOI: 10.1073/pnas.0912955107. PMID: 20194732
31. Gordon C. Thermal physiology of laboratory mice: defining thermoneutrality. J. Thermal. Biology. 2012; 37: 654-685.
32. Karp C.L. Unstressing intemperate models: how cold stress undermines mouse modeling. J. Exp. Med. 2012; 209 (6): 1069-1074. DOI: 10.1084/jem.20120988. PMID: 22665703
33. Jiang Q., Cross A.S., Singh I.S., Chen T.T., Viscardi R.M., Hasday J.D. Febrile core temperature is essential for optimal host defense in bacterial peritonitis. Infect. Immun. 2000; 68 (3): 1265-1270. DOI: 10.1128/IAI.68.3.1265-1270.2000. PMID: 10678936
34. Mestas J., Hughes C.C. Of mice and not men: differences between mouse and human immunology. J. Immunol. 2004; 172 (5): 2731-2738. DOI: 10.4049/jimmunol.172.5.2731. PMID: 14978070
35. Ong G.L., Mattes M.J. Mouse strains with typical mammalian levels of complement activity. J. Immunol. Methods. 1989; 125 (1-2): 147-158. DOI: 10.1016/0022-1759(89)90088-4. PMID: 2607149
36. Ratelade J., Verkman A.S. Inhibitor(s) of the classical complement pathway in mouse serum limit the utility of mice as experimental models of neuromyelitis optica. Mol. Immunol. 2014; 62 (1): 104-113. DOI: 10.1016/j.molimm.2014.06.003. PMID: 24980869
37. Limaye A.P., Kirby K.A., Rubenfeld G.D., Leisenring W.M., Bulger E.M., Neff M.J., Gibran N.S., Huang M.L., Santo Hayes T.K., Corey L., Boeckh M. Cytomegalovirus reactivation in critically ill immunocompetent patients. JAMA. 2008; 300 (4): 413-422. DOI: 10.1001/jama.300.4.413. PMID: 18647984
38. Luyt C.E., Combes A., Deback C., Aubriot-Lorton M.H., Nieszkowska A., Trouillet J.L., Capron F., Agut H., Gibert C., Chastre J. Herpes simplex virus lung infection in patients undergoing prolonged mechanical ventilation. Am. J. Respir. Crit. Care Med. 2007; 175 (9): 935-942. DOI: 10.1164/rccm.200609-1322OC. PMID: 17234903
39. Alonso J.M., Guiyoule A., Zarantonelli M.L., Ramisse F., Pires R., Antignac A., Deghmane A.E., Huerre M., van der Werf S., Taha M.K. A model of meningococcal bacteremia after respiratory superinfection in influenza a virus-infected mice. FEMS Microbiol Lett. 2003; 222 (1): 99-106. DOI: 10.1016/S0378-1097(03)00252-0. PMID: 12757952
40. Nansen A., Christensen J.P., Marker O., Thomsen A.R. Sensitization to lipopolysaccharide in mice with asymptomatic viral infection: role of T cell-dependent production of interferon-gamma. J. Infect. Dis. 1997; 176 (1): 151-157. DOI: 10.1086/514017. PMID: 9207361
41. Speshock J.L., Doyon-Reale N., Rabah R., Neely M.N., Roberts P.C. Filamentous influenza A virus infection predisposes mice to fatal septicemia following superinfection with Streptococcus pneumoniae serotype 3. Infect. Immun. 2007; 75: 3102-3111. DOI: 10.1128/IAI.01943-06. PMID: 17403870
42. Salgado-Pabón W., Breshears L., Spaulding A.R., Merriman J.A., Stach C.S., Horswill A.R., Peterson M.L., Schlievert P.M. Superantigens are critical for Staphylococcus aureus infective endocarditis, sepsis, and acute kidney injury. MBio. 2013; 4 (4): pii: e00494-13. DOI: 10.1128/mBio.00494-13. PMID: 23963178
43. Fairbairn L., Kapetanovic R., Beraldi D., Sester D.P., Tuggle C.K., Archibald A.L., Hume D.A. Comparative analysis of monocyte subsets in the pig. J. Immunol. 2013; 190 (12): 6389-6396. DOI: 10.4049/jimmunol.1300365. PMID: 23667115
44. Kapetanovic R., Fairbairn L., Beraldi D., Sester D.P., Archibald A.L., Tuggle C.K., Hume D.A. Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide. J. Immunol. 2012; 188 (7): 3382-3394. DOI: 10.4049/jimmunol.1102649. PMID: 22393154
45. Barreiro L.B., Marioni J.C., Blekhman R., Stephens M., Gilad Y. Functional comparison of innate immune signaling pathways in primates. PLoS Genet. 2010; 6 (12): e1001249. DOI: 10.1371/journal.pgen.1001249. PMID: 21187902
46. Brinkworth J.F., Pechenkina E.A., Silver J., Goyert S.M. Innate immune responses to TLR2 and TLR4 agonists differ between baboons, chimpanzees and humans. J. Med. Primatol. 2012; 41 (6): 388-393. DOI: 10.1111/jmp.12002. PMID: 22978822
47. Melican K., Michea Veloso P., Martin T., Bruneval P., Duménil G. Adhesion of Neisseria meningitidis to dermal vessels leads to local vascular damage and purpura in a humanized mouse model. PLoS Pathog. 2013; 9 (1): e1003139. DOI: 10.1371/journal.ppat.1003139. PMID: 23359320
48. Rongvaux A., Willinger T., Martinek J., Strowig T., Gearty S.V., Teichmann L.L., Saito Y., Marches F., Halene S., Palucka A.K., Manz M.G., Flavell R.A. Development and function of human innate immune cells in a humanized mouse model. Nat. Biotechnol. 2014; 32 (4): 364-372. DOI: 10.1038/nbt.2858. PMID: 24633240
49. Huh D., Kim H.J., Fraser J.P., Shea D.E., Khan M., Bahinski A., Hamilton G.A., Ingber D.E. Microfabrication of human organs-on-chips. Nat. Protoc. 2013; 8 (11): 2135-2157. DOI: 10.1038/nprot.2013.137. PMID: 24113786
50. Lancaster M.A., Knoblich J.A. Organogenesis in a dish: modeling development and disease using organoid technologies. Science. 2014; 345 (6194): 1247125. DOI: 10.1126/science.1247125. PMID: 25035496
51. Zhang Y.G., Wu S., Xia Y., Sun J. Salmonella-infected crypt-derived intestinal organoid culture system for host-bacterial interactions. Physiol. Rep. 2014; 2 (9): pii: e12147. DOI: 10.14814/phy2.12147. PMID: 25214524
52. Efron P.A., Mohr A.M., Moore F.A., Moldawer L.L. The future of murine sepsis and trauma research models. J. Leukoc. Biol. 2015; 98 (6): 945-952. DOI: 10.1189/jlb.5MR0315-127R. PMID: 26034205
53. Hotchkiss R.S., Monneret G., Payen D. Sepsis-induced immunosuppression: from cellular dysfunctions to immunotherapy. Nat. Rev. Immunol. 2013; 13 (12): 862-874. DOI: 10.1038/nri3552. PMID: 24232462
54. Suzuki T., Shimizu T., Szalay L., Choudhry M.A., Rue L.W.3rd, Bland K.I., Chaudry I.H. Androstenediol ameliorates alterations in immune cells cytokine production capacity in a two-hit model of trauma-hemorrhage and sepsis. Cytokine. 2006; 34 (1-2): 76-84. DOI: 10.1016/j.cyto.2006.04.007. PMID: 16737821
55. Cavaillon J.M., Eisen D., Annane D. Is boosting the immune system in sepsis appropriate? Crit. Care. 2014; 18 (2): 216-225. DOI: 10.1186/cc13787. PMID: 24886820
Рецензия
Для цитирования:
Кавайон Ж. Новые методы лечения при сепсисе: модели на животных «не работают» (обзор). Общая реаниматология. 2018;14(3):46-53. https://doi.org/10.15360/1813-9779-2018-3-46-53
For citation:
Cavaillon J. New Approaches to Treat Sepsis: Animal Models «Do Not Work» (Review). General Reanimatology. 2018;14(3):46-53. https://doi.org/10.15360/1813-9779-2018-3-46-53
Просмотров: 685
Послать статью по эл. почте 