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Prospects for Using Atomic Power Microscopic Methods in Reanimatology

https://doi.org/10.15360/1813-9779-2008-4-51

Abstract

Objective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a pulsed electric field, by inducing erythrocytic membrane elec-troporation. Blood smears were obtained by the standard method. Images of the form and surface of individual erythrocytes and their enlarged fragments were obtained by means of a Femto Scan APM. The diameters, depths, and configuration of some pores were analyzed. This example and others were used to show a wide field and prospects for using an APM in reanimatology.

References

1. Яминский И. В.

2. Мороз В. В., Козлова Е. К., Богушевич М. С и соавт.Перфторан в суспензии крови. Эффекты закрепляющего и разрушающего действия на модифицированные электрическими импульсами мембраны. Общая реаниматология 2005; 1 (3): 5—10.

3. Tekle E., Astumian R. D., Friauf W. A., Chock P. B.Asymmetric pore distribution and loss of membrane lipid in electroporated DOPC vesicles. Biophys. J. 2001; 81 (8): 960—968.

4. Козлова Е. К., Мороз В. В., Богушевич М. С.,Черныш А. М.Влияние формы электрического импульса на электропорацию мембран эритроцитов. Общая реаниматология 2005; 1 (1): 42—47.

5. Chang D. C., Reese T. S.Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy. Biophys.J. 1990; 58 (7): 01—12.

6. Мороз В. В., Богушевич М. С., Черныш А. М. и соавт.Способ определения защиты мембран эритроцитов крови от воздействия пробойным импульсным электрическим полем. Патент на изобретение 2004; 228309

7. Черняев А. П., Черныш А. М., Алексеева П. Ю. и соавт.Диагностика скрытых повреждений мембран эритроцитов в результате воздействия физико-химических факторов. Технологии живых систем 2007; 4 (1): 28—36.

8. Филонов А. С., Яминский И. В.Обработка и анализ данных в сканирующей зондовой микроскопии: Алгоритмы и методы. Наноиндус-трия 2007; 2: 32—34.

9. Nowakowki R., Luckham P., Winlove P.Imaging erythrocytes under physiological conditions by atomic force microscopy. Biochimica et Biophysica Acta 2001; 1514: 170—176.

10. Gould S. A. C., Drake B., Prater C. B. et al.From atoms to integrated circuit chips, blood cells, and bacteria with the atomic force microscope. J. Vacuum S. and Technology 1990; A 8: 369—373.

11. Butt H-J., Wolff E. K., Gould S. A. C. et al.Imaging cells with the atomic force microscope. J. Structural Biology 1990; 105: 54—61.

12. Shroff S. G., Saner D. R., Lal R.Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy. Amer. J. Physiology 1995; 269 (C(1p+1)): C286—C292.

13. Hofmann U. G., Rotsch C., Parak W. J., Radmacher M.Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope. J. Structural Biology 1997; 119: 84—91.

14. Mathur A. B., Truskey G. A., Reichert W. M.Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells. Biophysical J. 2000; 78: 1725—1735.

15. Rosenbluth M. J., Lam W. A., Fletcher D. A.Force microscopy of nonad-herent cells: a comparison of leukemia cell deformability. Biophysical J. 2006; 90: 2994—3003.

16. Tokumasu F.,Jin A.J., DvorakJ. A.Lipid membrane phase behaviour elucidated in real time by controlled environment atomic force microscopy. J. Electron Microscopy 2002; 51 (1): 1—9.


Review

For citations:


Moroz V.V., Chernysh A.M., Yaminsky I.V., Kozlova Ye.K., Kiselev G.A., Filonov A.S., Bogushevich M.S., Gudkova O.Ye. Prospects for Using Atomic Power Microscopic Methods in Reanimatology. General Reanimatology. 2008;4(4):51. (In Russ.) https://doi.org/10.15360/1813-9779-2008-4-51

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ISSN 1813-9779 (Print)
ISSN 2411-7110 (Online)