IMPACT OF HYPERBARIC OXYGENATION ON RENAL AMMONIA EXCRETION DURING EXPERIMENTAL LIVER RESECTION
https://doi.org/10.15360/1813-9779-2015-2-56-63
Abstract
Objective: to study the impact of hyperbaric oxygenation (HBO) on renal ammonia excretion during liver resection (LR). Material and methods. Experiments were carried out on 138 pubescent rats (females). Three HBO sessions were performed at 3 ATA lasting 50 min once daily after LR (15—20% of the liver weight). The kidney, blood (aorta, v. renalis), and urine were examined. The levels of ammonia, glutamine, and urea were measured. Results. By eliminating postoperative arterial hyperammonemia, HBO stimulates urinary ammonia ion excretion. This is achieved through elimination of the inhibitory effect of LR under HBO on ammonia secretion into the renal tubules and through activation of intracellular ammoniagenesis in nephrocytes, including that uncoupled with arterial glutamine deamidation. HBO potentiates simultaneously the activating effect of LR on the glutamine cycle in the kidneys mainly through the hyperproduction of renal glutamine and its influx from them into the bloodstream. HPO enhances the stimulatory effect of LR on urea reabsorption in the kidneys, but, unlike non-oxygenated rats, there was no reduction in urine urea excretion due to increased arterial blood urea concentrations. Termination of the body's exposure to hyperoxia restores urea reabsorption in the kidneys with the persisting stimulatory impact of HBO on the formation of urea by nephrocytes and its incretion from the latter into the bloodstream.
About the Authors
P. N. Savilov
N.N. Burdenko Voronezh State Medical Academy, Voronezh, Russia 10, Studencheskaya St., Voronezh 394036
Tambov Central Republican Hospital, Tambov, Russia 4, Polevaya St., Pokrovo Prigorodnoe Rural Settlement, Tambov Region
Russian Federation
Russian Federation
D. V. Molchanov
N.N. Burdenko Voronezh State Medical Academy, Voronezh, Russia 10, Studencheskaya St., Voronezh 394036
Research Institute of Clinical Pediatrics, N.I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia
2, Taldomskaya St., Moscow 125412
Russian Federation
Russian Federation
References
1. Orlov Yu.P., Govorova N.V. Rol suktsinatov pri kriticheskikh sostoyaniyakh. Obshchaya Reanimatologiya. [Role of succinates in critical conditions. General Reanimatology]. 2014; 10 (6): 65—78. http://dx.doi.org/10.15360/1813-9779-2014-6-65-82. [In Russ.]
2. Tabakyan E.A., Partigulov S.A., Lepilin M.G., Burmistrova I.V., Vodyasov
3. V.D., Kotkina T.I., Titov V.N. Rannyaya diagnostika ostrogo povrezh deniya pochek pri operatsiyakh na otkrytom serdtse s iskusstvennym krovoobrashcheniem. Obshchaya Reanimatologiya. [Early diagnosis of acute kidney injury during open heart surgery under extracorporeal circulation. General Reanimatology]. 2013; 9 (1): 51—57. http://dx. doi.org/10.15360/1813-9779-2013-1-51. [In Russ.]
4. Kolesnikov S.V., Borisov A.S., Kornilov I.A., Lomivorotov V.V. Postoyannaya zamestitelnaya pochechnaya terapiya s ekstrakorporalnoi membrannoi oksigenatsiei v kardiokhirurgii. Obshchaya Reanimatologiya. [Continuous renal replacement therapy and extracorporeal membrane oxygenation in cardias surgery. General Reanimatology]. 2014; 10 (3): 75—84. http://dx.doi.org/10.15360/ 1813-9779-2014-3-75-84. [In Russ.]
5. Detyen P. Funktsiya pochek. V kn.:Shmidt R., Tevs G. (red.). Fiziologiya cheloveka. [Kidney function.In: Shmidt R., Tevs G. (eds.). Human physiology]. Moscow: Mir; 2010:3, 785—812. [In Russ.]
6. Curthoys N.P. Role of mitochondrial glutaminase in rat renal glutamine metabolism. J. Nutr. 2001; 131 (9 Suppl): 2491S—2497S. PMID: 11533299
7. Savilov P.N., Molchanov D.V. Kinetika ammiaka v organizme pri rezektsii pecheni i giperbaricheskoi oksigenatsii. [Kinetics of ammonia in the organism after liver resection and the hyperbaric oxygenation]. Zagalna Patologiya ta Patologichna Fiziologiya. 2010; 5 (3): 114—118. [In Russ.]
8. Kosenko E.A., Kaminsky Yu.G. Kletochnye mekhanizmy toksichnosti ammiaka. [Cellular mechanisms of toxicity of ammonia]. Moscow: izd-vo LKI; 2008. [In Russ.]
9. Savilov P.N., Molchanov D.V., Yakovlev V.N. Kinetika azotistykh metaboli tov v pochkakh pri rezektsii pecheni. [Kinetics of nitrous metabolites in the kidneys during liver resection]. Voprosy Biologicheskoi, Meditsinskoi i Farmatsevticheskoi Khimii. 2012; 10 (11): 43—47. [In Russ.]
10. Molchanov D.V., Savilov P.N. Pochechnye mekhanizmy eliminatsii ammiaka pri rezektsii pecheni (eksperimentalnoe issledovanie). [Renal mechanisms elimination of ammonia liver resections (experimental study)]. Rossiisky Vestnik Detskoi Khirurgii, Anesteziologii i Reanimatologii. 2013; 3 (3): 64—68. [In Russ.]
11. Leonov A.N. Giperoksiya. Adaptatsiya. Sanogenez. [Hyperoxia. Adaptation. Sanogenesis]. Voronezh: VGMA; 2006. [In Russ.]
12.
13. Savilov P.N., Yakovlev V.N. Hyperbaric oxygenation and partial hepatectomy effects on liver bloodstream, oxygen pressure and ammonia detoxication in the liver under chronic hepatitis. Iugosl. Physiol. Pharmacol. Acta. 2006; 42 (32): 103—114.
14. Savilov P.N. Rol i mesto giperbaricheskoi oksigenatsii pri pechenochnoi nedostatochnosti. Obshchaya Reanimatologiya. [Role and place of hyperbaric oxygenation in hepatic failure. General Reanimatology]. 2009; 5 (5): 72—79. http://dx.doi.org/10.15360/1813977920095
15. [In Russ.]13.Savilov P.N. Vliyanie giperbaricheskoi oksigenatsii na azotistyi balans organov zheludochnokishechnogo trakta pri rezektsii pecheni (eksperimentalnoe issledovanie. [Impact of hyperbaric oxygenation on nitric balance of gastrointestinal organs at hepatectomy: experimental study)]. Anesteziologiya i Reanimatologiya. 2005; 6: 59—62. PMID: 16499110. [In Russ.]
16. Savilov P.N. Azotistyi metabolism selezenki pri rezektsii pecheni i giperbaricheskoi oksigenatsii. [Nitric metabolism of the spleen during liver resection and hyperbaric oxygenation]. Biologichesky Zhurnal Armenii. 2014; 66 (2): 6—11. [In Russ.]
17. Silakova A.I., Trubin G.P., Yavlikova A.I. Mikrometod opredeleniya ammiaka i glutamine v tkanevykh trikhloruksusnykh ekstraktakh. [A micromethod of ammonia and glutamine in trichloroacetic acid tissue extracts]. Voprosy Meditsinskoi Khimii. 1962; 8 (5): 538—544. PMID: 13992815. [In Russ.]
18. Keller H., MüllerBeissenhirtz W., Neumann E. Eine methode zur ammoni akbestimmung in capillarblut. Klin. Wochenschr. 1967; 45 (6): 314—316. http://dx.doi.org/10.1007/BF01747105. PMID: 5588009
19. Harris M.M., Roth R.T., Harris R.S. Studies regenerating a glutamine like substance in blood and spinal fluid, including a method for its quantitative determination. J. Clin. Invest. 1943; 22 (4): 569—576. PMID: 16695039
20. Richterrich D. Clinical chemistry. N.Y.: Academia Press; 1962.
21. Savilov P.N. Glutaminovyi tsikl pecheni posle ee rezektsii. [Liver glutamic cycle after resection]. Voprosy Biologicheskoi, Meditsinskoi i Farmatsevticheskoi Khimii. 2010; 8 (7): 53—58. [In Russ.]
22. Lomivorotov V.V., Efremov S.M., Shmyrev V.A., Ponomarev D.N., Svyatchenko A.V., Knyazkova L.G. Kardioprotektornye effekty glutamine u patsientov s ishemicheskoi boleznyu serdtsa, operirovannykh v usloviyakh IK. [Cardioprotective effects of glutamine in patients with ischemic heart disease operated under conditions of extracorporeal blood circulation]. Anesteziologiya i Reanimatologiya. 2012; 2: 14—18. PMID: 22834281. [In Russ.]
23. Savilov P.N., Molchanov D.V., Yakovlev V.N. Vliyanie giperbaricheskoi oksigenatsii na kinetiku glutamine v organizme pri pechenochnoi nedostatochnosti. Obshchaya Reanimatologiya. [Impact of hyperbaric oxygenation on body glutamine kinetics in hepatic failure. General Reanimatology]. 2012; 8 (2): 20—27. http://dx.doi.org/10.15360/ 1813-9779-2012-2-20. [In Russ.]
24. Verbitskaya V.S., Dolgikh V.T., Korpacheva O.V., Ostroglyadova I.A. Korrektsiya glutaminom morfofunktsionalnykh narushenii tonkoi kishki i pecheni v posttravmaticheskom periode ushiba serdtsa (eksperimentalnoe issledovanie). Obshchaya Reanimatologiya. [Glutamine correction of morphofunctional disorders of the small bowel and liver in the posttraumatic period of experimental cardiac contusion (an experimental study). General Reanimatology]. 2014; 10 (2): 31—40. http://dx.doi.org/10.15360/1813-9779-2014-2-31-40. [In Russ.]
25.
Review
For citations:
Savilov P.N., Molchanov D.V. IMPACT OF HYPERBARIC OXYGENATION ON RENAL AMMONIA EXCRETION DURING EXPERIMENTAL LIVER RESECTION. General Reanimatology. 2015;11(2):56-63. https://doi.org/10.15360/1813-9779-2015-2-56-63
Views:
1369
Email this article 